Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy

Dongeun Kim, Wonsang Hwang, Youngjae Won, Sucbei Moon, Dug Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The Analog mean delay (AMD) method is a multiphoton detection fluorescence lifetime measurement method. We have investigated the effect of the linearity of a photodetector response on the performance of the AMD method, which is a multiphoton detected fluorescence lifetime measurement method. A Monte-Carlo simulation scheme was adapted to generate various electronic signals for the AMD method with different linearity conditions of a photodetector. It is found that the photon economy is better if a photodetector with better linearity is used especially when the number of detected photons per pulse is low.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XVIII
EditorsAmmasi Periasamy, Xiaoliang S. Xie, Xiaoliang S. Xie, Karsten Konig, Peter T. C. So
PublisherSPIE
ISBN (Electronic)9781510614819
DOIs
Publication statusPublished - 2018 Jan 1
EventMultiphoton Microscopy in the Biomedical Sciences XVIII 2018 - San Francisco, United States
Duration: 2018 Jan 282018 Jan 30

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10498
ISSN (Print)1605-7422

Other

OtherMultiphoton Microscopy in the Biomedical Sciences XVIII 2018
CountryUnited States
CitySan Francisco
Period18/1/2818/1/30

Fingerprint

Optical Imaging
economy
Photodetectors
Photons
linearity
photometers
Microscopy
Microscopic examination
Fluorescence
analogs
microscopy
Imaging techniques
life (durability)
fluorescence
augmentation
photons
pulses
electronics
simulation

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Kim, D., Hwang, W., Won, Y., Moon, S., & Kim, D. Y. (2018). Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy. In A. Periasamy, X. S. Xie, X. S. Xie, K. Konig, & P. T. C. So (Eds.), Multiphoton Microscopy in the Biomedical Sciences XVIII [104982G] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10498). SPIE. https://doi.org/10.1117/12.2290059
Kim, Dongeun ; Hwang, Wonsang ; Won, Youngjae ; Moon, Sucbei ; Kim, Dug Young. / Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy. Multiphoton Microscopy in the Biomedical Sciences XVIII. editor / Ammasi Periasamy ; Xiaoliang S. Xie ; Xiaoliang S. Xie ; Karsten Konig ; Peter T. C. So. SPIE, 2018. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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abstract = "The Analog mean delay (AMD) method is a multiphoton detection fluorescence lifetime measurement method. We have investigated the effect of the linearity of a photodetector response on the performance of the AMD method, which is a multiphoton detected fluorescence lifetime measurement method. A Monte-Carlo simulation scheme was adapted to generate various electronic signals for the AMD method with different linearity conditions of a photodetector. It is found that the photon economy is better if a photodetector with better linearity is used especially when the number of detected photons per pulse is low.",
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Kim, D, Hwang, W, Won, Y, Moon, S & Kim, DY 2018, Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy. in A Periasamy, XS Xie, XS Xie, K Konig & PTC So (eds), Multiphoton Microscopy in the Biomedical Sciences XVIII., 104982G, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10498, SPIE, Multiphoton Microscopy in the Biomedical Sciences XVIII 2018, San Francisco, United States, 18/1/28. https://doi.org/10.1117/12.2290059

Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy. / Kim, Dongeun; Hwang, Wonsang; Won, Youngjae; Moon, Sucbei; Kim, Dug Young.

Multiphoton Microscopy in the Biomedical Sciences XVIII. ed. / Ammasi Periasamy; Xiaoliang S. Xie; Xiaoliang S. Xie; Karsten Konig; Peter T. C. So. SPIE, 2018. 104982G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10498).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Kim D, Hwang W, Won Y, Moon S, Kim DY. Enhancement of measurement speed and photon economy in multiphoton detected fluorescence lifetime imaging microscopy. In Periasamy A, Xie XS, Xie XS, Konig K, So PTC, editors, Multiphoton Microscopy in the Biomedical Sciences XVIII. SPIE. 2018. 104982G. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2290059