Enhancement of the efficiency of femto-second optical transfection

Bavishna B. Praveen, David Stevenson, Maciej Antkowiak, Frank J. Gunn-Moore, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cell transfection is the process in which extra cellular nucleic acids such as DNA, RNA, Si-RNA can be deliberately injected into the cytoplasm of the cell. This technique of cell transfection forms a central tool in the hands of a cell biologist to explore the mechanism within the cell. in optical transfection a well focused laser spot alters the permeability of the cell membrane so as to allow the entry of extra-nuclear materials into the cell. Femto-second optical transfection have proved to be better than other laser based cell transfection, owing to the three dimensionally confined multi-photon effects on the cell membrane thereby leaving the rest of the cell unaffected. Even though the femto-second optical transfection has proved to be sterile, non-invasive and highly selective, it has to improve in terms of efficiency, and throughput to address real life problems. We report here a method to achieve significant enhancement in the efficiency of femto-second optical transfection. The protocol of the transfection procedure is modified by adding a suitable biochemical reagent - Nupherin-neuron - into the cell medium during the transfection, which can assist the delivery of DNA into the nucleus once the DNA gets injected into the cytoplasm of the cell. We achieved a 3 fold enhancement in the transfection efficiency with this modified protocol. Also we report for the first time the transfection of recently trypsinised cells with a very high transfection efficiency, which would pave way to the development of high throughput microfluidic optical transfection devices.

Original languageEnglish
Title of host publicationPhotonics 2010
Subtitle of host publicationTenth International Conference on Fiber Optics and Photonics
DOIs
Publication statusPublished - 2011 Sep 14
EventPhotonics 2010: 10th International Conference on Fiber Optics and Photonics - Guwahati, India
Duration: 2010 Dec 112010 Dec 15

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8173
ISSN (Print)0277-786X

Conference

ConferencePhotonics 2010: 10th International Conference on Fiber Optics and Photonics
CountryIndia
CityGuwahati
Period10/12/1110/12/15

Fingerprint

Femtosecond
Enhancement
DNA
augmentation
Cell
Cell membranes
cells
RNA
Throughput
Lasers
Nucleic acids
Optical devices
Microfluidics
Nucleic Acids
Neurons
deoxyribonucleic acid
cytoplasm
Photons
Membrane
Laser

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Praveen, B. B., Stevenson, D., Antkowiak, M., Gunn-Moore, F. J., & Dholakia, K. (2011). Enhancement of the efficiency of femto-second optical transfection. In Photonics 2010: Tenth International Conference on Fiber Optics and Photonics [817307] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8173). https://doi.org/10.1117/12.899117
Praveen, Bavishna B. ; Stevenson, David ; Antkowiak, Maciej ; Gunn-Moore, Frank J. ; Dholakia, Kishan. / Enhancement of the efficiency of femto-second optical transfection. Photonics 2010: Tenth International Conference on Fiber Optics and Photonics. 2011. (Proceedings of SPIE - The International Society for Optical Engineering).
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Praveen, BB, Stevenson, D, Antkowiak, M, Gunn-Moore, FJ & Dholakia, K 2011, Enhancement of the efficiency of femto-second optical transfection. in Photonics 2010: Tenth International Conference on Fiber Optics and Photonics., 817307, Proceedings of SPIE - The International Society for Optical Engineering, vol. 8173, Photonics 2010: 10th International Conference on Fiber Optics and Photonics, Guwahati, India, 10/12/11. https://doi.org/10.1117/12.899117

Enhancement of the efficiency of femto-second optical transfection. / Praveen, Bavishna B.; Stevenson, David; Antkowiak, Maciej; Gunn-Moore, Frank J.; Dholakia, Kishan.

Photonics 2010: Tenth International Conference on Fiber Optics and Photonics. 2011. 817307 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8173).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Praveen BB, Stevenson D, Antkowiak M, Gunn-Moore FJ, Dholakia K. Enhancement of the efficiency of femto-second optical transfection. In Photonics 2010: Tenth International Conference on Fiber Optics and Photonics. 2011. 817307. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.899117