Enhancement of the safety of live influenza vaccine by attenuating mutations from cold-adapted hemagglutinin

Yoon Jae Lee, Yo Han Jang, Paul Kim, Yun Ha Lee, Young Jae Lee, Young Ho Byun, Kwang Hee Lee, Kyusik Kim, Baik Lin Seong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In our previous study, X-31ca-based H5N1 LAIVs, in particular, became more virulent in mice than the X-31ca MDV, possibly by the introduction of the surface antigens of highly pathogenic H5N1 influenza virus, implying that additional attenuation is needed in this cases to increase the safety level of the vaccine. In this report we suggest an approach to further increase the safety of LAIV through additional cold-adapted mutations in the hemagglutinin. The cold-adaptation of X-31 virus resulted in four amino acid mutations in the HA. We generated a panel of 7:1 reassortant viruses each carrying the hemagglutinins with individual single amino acid mutations. We examined their phenotypes and found a major attenuating mutation, N81K. This attenuation marker conferred additional temperature-sensitive and attenuation phenotype to the LAIV. Our data indicate that the cold-adapted mutation in the HA confers additional attenuation to the LAIV strain, without compromising its productivity and immune response.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalVirology
Volume491
DOIs
Publication statusPublished - 2016 Apr 1

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Influenza Vaccines
Hemagglutinins
Safety
Mutation
Reassortant Viruses
H5N1 Subtype Influenza A Virus
Phenotype
Amino Acids
Surface Antigens
Orthomyxoviridae
Vaccines
Viruses
Temperature

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Lee, Yoon Jae ; Jang, Yo Han ; Kim, Paul ; Lee, Yun Ha ; Lee, Young Jae ; Byun, Young Ho ; Lee, Kwang Hee ; Kim, Kyusik ; Seong, Baik Lin. / Enhancement of the safety of live influenza vaccine by attenuating mutations from cold-adapted hemagglutinin. In: Virology. 2016 ; Vol. 491. pp. 1-9.
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Enhancement of the safety of live influenza vaccine by attenuating mutations from cold-adapted hemagglutinin. / Lee, Yoon Jae; Jang, Yo Han; Kim, Paul; Lee, Yun Ha; Lee, Young Jae; Byun, Young Ho; Lee, Kwang Hee; Kim, Kyusik; Seong, Baik Lin.

In: Virology, Vol. 491, 01.04.2016, p. 1-9.

Research output: Contribution to journalArticle

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AU - Kim, Kyusik

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