Enhancing proliferation and optimizing the culture condition for human bone marrow stromal cells using hypoxia and fibroblast growth factor-2

Jung Seok Lee, Seul Ki Kim, Byung Joo Jung, Seong Bok Choi, Eun Young Choi, ChangSung Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study aimed to determine the cellular characteristics and behaviors of human bone marrow stromal cells (hBMSCs) expanded in media in a hypoxic or normoxic condition and with or without fibroblast growth factor-2 (FGF-2) treatment. hBMSCs isolated from the vertebral body and expanded in these four groups were evaluated for cellular proliferation/migration, colony-forming units, cell-surface characterization, in vitro differentiation, in vivo transplantation, and gene expression. Culturing hBMSCs using a particular environmental factor (hypoxia) and with the addition of FGF-2 increased the cellular proliferation rate while enhancing the regenerative potential, modulated the multipotency-related processes (enhanced chondrogenesis-related processes/osteogenesis, but reduced adipogenesis), and increased cellular migration and collagen formation. The gene expression levels in the experimental samples showed activation of the hypoxia-inducible factor-1 pathway and glycolysis in the hypoxic condition, with this not being affected by the addition of FGF-2. The concurrent application of hypoxia and FGF-2 could provide a favorable condition for culturing hBMSCs to be used in clinical applications associated with bone tissue engineering, due to the enhancement of cellular proliferation and regenerative potential.

Original languageEnglish
Pages (from-to)87-95
Number of pages9
JournalStem Cell Research
Volume28
DOIs
Publication statusPublished - 2018 Apr 1

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Cell Hypoxia
Fibroblast Growth Factor 2
Mesenchymal Stromal Cells
Cell Proliferation
Gene Expression
Chondrogenesis
Hypoxia-Inducible Factor 1
Adipogenesis
Glycolysis
Tissue Engineering
Osteogenesis
Collagen
Stem Cells
Transplantation
Bone and Bones
Hypoxia

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Lee, Jung Seok ; Kim, Seul Ki ; Jung, Byung Joo ; Choi, Seong Bok ; Choi, Eun Young ; Kim, ChangSung. / Enhancing proliferation and optimizing the culture condition for human bone marrow stromal cells using hypoxia and fibroblast growth factor-2. In: Stem Cell Research. 2018 ; Vol. 28. pp. 87-95.
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Enhancing proliferation and optimizing the culture condition for human bone marrow stromal cells using hypoxia and fibroblast growth factor-2. / Lee, Jung Seok; Kim, Seul Ki; Jung, Byung Joo; Choi, Seong Bok; Choi, Eun Young; Kim, ChangSung.

In: Stem Cell Research, Vol. 28, 01.04.2018, p. 87-95.

Research output: Contribution to journalArticle

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