Enhancing trophic support of mesenchymal stem cells by ex vivo treatment with trophic factors

Yun Jung Choi, Wen Yu Li, Gyeong Joon Moon, Phil Hyu Lee, Young Hwan Ahn, Gwang Lee, Oh Young Bang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Several studies have examined the enhanced efficacy of mesenchymal stem cells (MSCs) using neurotrophic factor transfection in ischemic rat models. However, gene therapy, e.g., the application of MSCs transfected with neurotrophic factors, is not feasible in clinical practice for ethical reasons. Therefore, we evaluated cultivation with specific trophic factors in an attempt to enhance the efficacy of human MSCs (hMSCs) in ischemic stroke. Methods: Using quantitative sandwich enzyme-linked immunosorbent assay (ELISA), we analyzed the levels of trophic factors released from hMSCs after treatment with ischemic brain extract. Trophic factors were pretreated under ex vivo culture conditions. The concentrations of each trophic factor produced by the trophic factor-pretreated and non-pretreated hMSCs were then measured and compared. Results: hMSCs cultured with ischemic rat brain extract showed increased production of BDNF (brain-derived neurotrophic factor), VEGF (vascular endothelial growth factor) and HGF (hepatocyte growth factor). Ex vivo treatment with trophic factors led to a further increase in the production of the trophic factor by hMSC, suggesting autocrine regulation of hMSCs. The morphology and expression of surface markers of hMSCs were not changed, but the cell viability and cell proliferation ability increased after treatment with trophic factors. Conclusions: Our data indicate that hMSCs provide trophic support to the ischemic brain, which can be enhanced by ex vivo treatment of trophic factors during cultivation of hMSCs.

Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalJournal of the Neurological Sciences
Volume298
Issue number1-2
DOIs
Publication statusPublished - 2010 Nov 15

Fingerprint

Mesenchymal Stromal Cells
Therapeutics
Nerve Growth Factors
Brain
Hepatocyte Growth Factor
Brain-Derived Neurotrophic Factor
Genetic Therapy
Vascular Endothelial Growth Factor A
Transfection
Cell Survival
Enzyme-Linked Immunosorbent Assay
Stroke
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Choi, Yun Jung ; Li, Wen Yu ; Moon, Gyeong Joon ; Lee, Phil Hyu ; Ahn, Young Hwan ; Lee, Gwang ; Bang, Oh Young. / Enhancing trophic support of mesenchymal stem cells by ex vivo treatment with trophic factors. In: Journal of the Neurological Sciences. 2010 ; Vol. 298, No. 1-2. pp. 28-34.
@article{71bdb94d963f43cebe0ef59e4de90baa,
title = "Enhancing trophic support of mesenchymal stem cells by ex vivo treatment with trophic factors",
abstract = "Background: Several studies have examined the enhanced efficacy of mesenchymal stem cells (MSCs) using neurotrophic factor transfection in ischemic rat models. However, gene therapy, e.g., the application of MSCs transfected with neurotrophic factors, is not feasible in clinical practice for ethical reasons. Therefore, we evaluated cultivation with specific trophic factors in an attempt to enhance the efficacy of human MSCs (hMSCs) in ischemic stroke. Methods: Using quantitative sandwich enzyme-linked immunosorbent assay (ELISA), we analyzed the levels of trophic factors released from hMSCs after treatment with ischemic brain extract. Trophic factors were pretreated under ex vivo culture conditions. The concentrations of each trophic factor produced by the trophic factor-pretreated and non-pretreated hMSCs were then measured and compared. Results: hMSCs cultured with ischemic rat brain extract showed increased production of BDNF (brain-derived neurotrophic factor), VEGF (vascular endothelial growth factor) and HGF (hepatocyte growth factor). Ex vivo treatment with trophic factors led to a further increase in the production of the trophic factor by hMSC, suggesting autocrine regulation of hMSCs. The morphology and expression of surface markers of hMSCs were not changed, but the cell viability and cell proliferation ability increased after treatment with trophic factors. Conclusions: Our data indicate that hMSCs provide trophic support to the ischemic brain, which can be enhanced by ex vivo treatment of trophic factors during cultivation of hMSCs.",
author = "Choi, {Yun Jung} and Li, {Wen Yu} and Moon, {Gyeong Joon} and Lee, {Phil Hyu} and Ahn, {Young Hwan} and Gwang Lee and Bang, {Oh Young}",
year = "2010",
month = "11",
day = "15",
doi = "10.1016/j.jns.2010.09.003",
language = "English",
volume = "298",
pages = "28--34",
journal = "Journal of the Neurological Sciences",
issn = "0022-510X",
publisher = "Elsevier",
number = "1-2",

}

Enhancing trophic support of mesenchymal stem cells by ex vivo treatment with trophic factors. / Choi, Yun Jung; Li, Wen Yu; Moon, Gyeong Joon; Lee, Phil Hyu; Ahn, Young Hwan; Lee, Gwang; Bang, Oh Young.

In: Journal of the Neurological Sciences, Vol. 298, No. 1-2, 15.11.2010, p. 28-34.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Enhancing trophic support of mesenchymal stem cells by ex vivo treatment with trophic factors

AU - Choi, Yun Jung

AU - Li, Wen Yu

AU - Moon, Gyeong Joon

AU - Lee, Phil Hyu

AU - Ahn, Young Hwan

AU - Lee, Gwang

AU - Bang, Oh Young

PY - 2010/11/15

Y1 - 2010/11/15

N2 - Background: Several studies have examined the enhanced efficacy of mesenchymal stem cells (MSCs) using neurotrophic factor transfection in ischemic rat models. However, gene therapy, e.g., the application of MSCs transfected with neurotrophic factors, is not feasible in clinical practice for ethical reasons. Therefore, we evaluated cultivation with specific trophic factors in an attempt to enhance the efficacy of human MSCs (hMSCs) in ischemic stroke. Methods: Using quantitative sandwich enzyme-linked immunosorbent assay (ELISA), we analyzed the levels of trophic factors released from hMSCs after treatment with ischemic brain extract. Trophic factors were pretreated under ex vivo culture conditions. The concentrations of each trophic factor produced by the trophic factor-pretreated and non-pretreated hMSCs were then measured and compared. Results: hMSCs cultured with ischemic rat brain extract showed increased production of BDNF (brain-derived neurotrophic factor), VEGF (vascular endothelial growth factor) and HGF (hepatocyte growth factor). Ex vivo treatment with trophic factors led to a further increase in the production of the trophic factor by hMSC, suggesting autocrine regulation of hMSCs. The morphology and expression of surface markers of hMSCs were not changed, but the cell viability and cell proliferation ability increased after treatment with trophic factors. Conclusions: Our data indicate that hMSCs provide trophic support to the ischemic brain, which can be enhanced by ex vivo treatment of trophic factors during cultivation of hMSCs.

AB - Background: Several studies have examined the enhanced efficacy of mesenchymal stem cells (MSCs) using neurotrophic factor transfection in ischemic rat models. However, gene therapy, e.g., the application of MSCs transfected with neurotrophic factors, is not feasible in clinical practice for ethical reasons. Therefore, we evaluated cultivation with specific trophic factors in an attempt to enhance the efficacy of human MSCs (hMSCs) in ischemic stroke. Methods: Using quantitative sandwich enzyme-linked immunosorbent assay (ELISA), we analyzed the levels of trophic factors released from hMSCs after treatment with ischemic brain extract. Trophic factors were pretreated under ex vivo culture conditions. The concentrations of each trophic factor produced by the trophic factor-pretreated and non-pretreated hMSCs were then measured and compared. Results: hMSCs cultured with ischemic rat brain extract showed increased production of BDNF (brain-derived neurotrophic factor), VEGF (vascular endothelial growth factor) and HGF (hepatocyte growth factor). Ex vivo treatment with trophic factors led to a further increase in the production of the trophic factor by hMSC, suggesting autocrine regulation of hMSCs. The morphology and expression of surface markers of hMSCs were not changed, but the cell viability and cell proliferation ability increased after treatment with trophic factors. Conclusions: Our data indicate that hMSCs provide trophic support to the ischemic brain, which can be enhanced by ex vivo treatment of trophic factors during cultivation of hMSCs.

UR - http://www.scopus.com/inward/record.url?scp=77958152038&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958152038&partnerID=8YFLogxK

U2 - 10.1016/j.jns.2010.09.003

DO - 10.1016/j.jns.2010.09.003

M3 - Article

C2 - 20864125

AN - SCOPUS:77958152038

VL - 298

SP - 28

EP - 34

JO - Journal of the Neurological Sciences

JF - Journal of the Neurological Sciences

SN - 0022-510X

IS - 1-2

ER -