Enterotoxigenic bacteroides fragilis infection exacerbates tumorigenesis in AOM/DSS mouse model

Soonjae Hwang, Chang Gun Lee, Minjeong Jo, Chan Oh Park, Sun Yeong Gwon, Samnoh Hwang, Hye Chin Yi, So Yeon Lee, Yong Bin Eom, Baktiar Karim, Ki Jong Rhee

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The azoxymethane (AOM)/dextran sulfate sodium (DSS) murine model is commonly used to study colitis-associated cancer. The human commensal bacterium, enterotoxigenic Bacteroides fragilis (ETBF) secretes the Bacteroides fragilis toxin (BFT) which is necessary and sufficient to cause colitis. We report that BALB/c mice infected with WT-ETBF and administered three cycles of AOM/DSS developed numerous, large-sized polyps predominantly in the colorectal region. In addition, AOM/DSS-treated BALB/c mice orally inoculated with wild-type nontoxigenic Bacteroides fragilis (WT-NTBF) overexpressing bft (rETBF) developed numerous polyps whereas mice infected with WT-NTBF overexpressing a biologically inactive bft (rNTBF) did not promote polyp formation. Unexpectedly, the combination of AOM+ETBF did not induce polyp formation whereas ETBF+DSS did induce polyp development in a subset of BALB/c mice. In conclusion, WT-ETBF promoted polyp development in AOM/DSS murine model with increased colitis in BALB/c mice. The model described herein provides an experimental platform for understanding ETBF-induced colonic tumorigenesis and studying colorectal cancer in wild-type mice.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalInternational Journal of Medical Sciences
Volume17
Issue number2
DOIs
Publication statusPublished - 2020

Bibliographical note

Funding Information:
Supplementary figures. http://www.medsci.org/v17p0145s1.pdf Acknowledgements This work was supported by NRF (National Research Foundation of Korea) Grant funded by the Ministry of Education (2017R1D1A1A02018088 and NRF-2017R1D1A1B03032960), and NRF-2017-Fostering Core Leaders of the Future Basic Science Program /Global Ph.D. Fellowship Program, 2017H1A2A10457 27. In addition, this work was supported in part by the Yonsei University Wonju Campus Future-Leading Research Initiative of 2017 (2017-52-0078). This work was supported in part by the Yonsei University Research Fund of 2019.

Funding Information:
This work was supported by NRF (National Research Foundation of Korea) Grant funded by the Ministry of Education (2017R1D1A1A02018088 and NRF-2017R1D1A1B03032960), and NRF-2017-Foster-ing Core Leaders of the Future Basic Science Program /Global Ph.D. Fellowship Program, 2017H1A2A10457 27. In addition, this work was supported in part by the Yonsei University Wonju Campus Future-Leading Research Initiative of 2017 (2017-52-0078). This work was supported in part by the Yonsei University Research Fund of 2019.

Publisher Copyright:
© The author(s).

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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