Abstract
The azoxymethane (AOM)/dextran sulfate sodium (DSS) murine model is commonly used to study colitis-associated cancer. The human commensal bacterium, enterotoxigenic Bacteroides fragilis (ETBF) secretes the Bacteroides fragilis toxin (BFT) which is necessary and sufficient to cause colitis. We report that BALB/c mice infected with WT-ETBF and administered three cycles of AOM/DSS developed numerous, large-sized polyps predominantly in the colorectal region. In addition, AOM/DSS-treated BALB/c mice orally inoculated with wild-type nontoxigenic Bacteroides fragilis (WT-NTBF) overexpressing bft (rETBF) developed numerous polyps whereas mice infected with WT-NTBF overexpressing a biologically inactive bft (rNTBF) did not promote polyp formation. Unexpectedly, the combination of AOM+ETBF did not induce polyp formation whereas ETBF+DSS did induce polyp development in a subset of BALB/c mice. In conclusion, WT-ETBF promoted polyp development in AOM/DSS murine model with increased colitis in BALB/c mice. The model described herein provides an experimental platform for understanding ETBF-induced colonic tumorigenesis and studying colorectal cancer in wild-type mice.
Original language | English |
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Pages (from-to) | 145-152 |
Number of pages | 8 |
Journal | International Journal of Medical Sciences |
Volume | 17 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2020 |
Bibliographical note
Funding Information:Supplementary figures. http://www.medsci.org/v17p0145s1.pdf Acknowledgements This work was supported by NRF (National Research Foundation of Korea) Grant funded by the Ministry of Education (2017R1D1A1A02018088 and NRF-2017R1D1A1B03032960), and NRF-2017-Fostering Core Leaders of the Future Basic Science Program /Global Ph.D. Fellowship Program, 2017H1A2A10457 27. In addition, this work was supported in part by the Yonsei University Wonju Campus Future-Leading Research Initiative of 2017 (2017-52-0078). This work was supported in part by the Yonsei University Research Fund of 2019.
Funding Information:
This work was supported by NRF (National Research Foundation of Korea) Grant funded by the Ministry of Education (2017R1D1A1A02018088 and NRF-2017R1D1A1B03032960), and NRF-2017-Foster-ing Core Leaders of the Future Basic Science Program /Global Ph.D. Fellowship Program, 2017H1A2A10457 27. In addition, this work was supported in part by the Yonsei University Wonju Campus Future-Leading Research Initiative of 2017 (2017-52-0078). This work was supported in part by the Yonsei University Research Fund of 2019.
Publisher Copyright:
© The author(s).
All Science Journal Classification (ASJC) codes
- Medicine(all)