Entrapment of enzyme-linked magnetic nanoparticles within poly(ethylene glycol) hydrogel microparticles prepared by photopatterning

Sami Park, Yeol Lee, Dae Nyun Kim, Sangphil Park, Eunji Jang, Won Gun Koh

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In this study, hydrogel microparticles containing enzyme-linked magnetic nanoparticles (MNPs) were prepared. Peroxidase (POD), a model enzyme, was covalently immobilized on the surface of MNPs using 3-aminopropyltriethoxysilane (APTES), and the resultant POD-linked MNPs were entrapped within various shapes of hydrogel microparticles using photopatterning. Pre-immobilizing POD on the MNP surface made it possible to use hydrogels prepared from high molecular weight PEG without enzyme leaching, which enhanced the reaction rate of entrapped enzymes by reducing resistance to mass transport. Quantitative assays showed a linear correspondence between fluorescence intensity and H2O2 concentrations below 15 mM.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalReactive and Functional Polymers
Volume69
Issue number5
DOIs
Publication statusPublished - 2009 May 1

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry
  • Biochemistry
  • Chemical Engineering(all)
  • Polymers and Plastics
  • Materials Chemistry

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