Enzymatic esterification of β-methylglucoside with acrylic/ methacrylic acid in organic solvents

Dae Won Park, Seungjoo Haam, Ik Sung Ahn, Tai Gyu Lee, Hae Sung Kim, Woo Sik Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The enzymatic esterifications of β-methylglucoside with acrylic acid/methacrylic acid were carried out using Novozym 435. t-Butanol indicating the highest conversion value was determined as an optimal solvent. The molar ratio (β-methylglucoside:acids) of 1:15 was most favorable to the esterification. The enzyme concentration of 5% (w/v), and the temperature (50°C for β-methylglucoside:acrylic acid, 45°C for β-methylglucoside:methacrylic acid) resulted in the highest final conversion. β-Methylglucoside of 60 gl-1 was found to be most effective in terms of short reaction time as well as product concentrations. Under these conditions, the maximum conversions for the esterification of β-methylglucoside with acrylic acid and β-methylglucoside with methacrylic acid were 59.3% after 12 h and 71.3% after 72 h, respectively. The structural analysis of the products was performed by FT-IR spectroscopy and 1H NMR.

Original languageEnglish
Pages (from-to)151-160
Number of pages10
JournalJournal of Biotechnology
Volume107
Issue number2
DOIs
Publication statusPublished - 2004 Jan 22

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Esterification
Organic solvents
Acrylics
Acids
tert-Butyl Alcohol
methacrylic acid
methylglucoside
acrylic acid
Butenes
Structural analysis
Infrared spectroscopy
Spectrum Analysis
Enzymes
Nuclear magnetic resonance
Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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title = "Enzymatic esterification of β-methylglucoside with acrylic/ methacrylic acid in organic solvents",
abstract = "The enzymatic esterifications of β-methylglucoside with acrylic acid/methacrylic acid were carried out using Novozym 435. t-Butanol indicating the highest conversion value was determined as an optimal solvent. The molar ratio (β-methylglucoside:acids) of 1:15 was most favorable to the esterification. The enzyme concentration of 5{\%} (w/v), and the temperature (50°C for β-methylglucoside:acrylic acid, 45°C for β-methylglucoside:methacrylic acid) resulted in the highest final conversion. β-Methylglucoside of 60 gl-1 was found to be most effective in terms of short reaction time as well as product concentrations. Under these conditions, the maximum conversions for the esterification of β-methylglucoside with acrylic acid and β-methylglucoside with methacrylic acid were 59.3{\%} after 12 h and 71.3{\%} after 72 h, respectively. The structural analysis of the products was performed by FT-IR spectroscopy and 1H NMR.",
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Enzymatic esterification of β-methylglucoside with acrylic/ methacrylic acid in organic solvents. / Park, Dae Won; Haam, Seungjoo; Ahn, Ik Sung; Lee, Tai Gyu; Kim, Hae Sung; Kim, Woo Sik.

In: Journal of Biotechnology, Vol. 107, No. 2, 22.01.2004, p. 151-160.

Research output: Contribution to journalArticle

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AU - Park, Dae Won

AU - Haam, Seungjoo

AU - Ahn, Ik Sung

AU - Lee, Tai Gyu

AU - Kim, Hae Sung

AU - Kim, Woo Sik

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AB - The enzymatic esterifications of β-methylglucoside with acrylic acid/methacrylic acid were carried out using Novozym 435. t-Butanol indicating the highest conversion value was determined as an optimal solvent. The molar ratio (β-methylglucoside:acids) of 1:15 was most favorable to the esterification. The enzyme concentration of 5% (w/v), and the temperature (50°C for β-methylglucoside:acrylic acid, 45°C for β-methylglucoside:methacrylic acid) resulted in the highest final conversion. β-Methylglucoside of 60 gl-1 was found to be most effective in terms of short reaction time as well as product concentrations. Under these conditions, the maximum conversions for the esterification of β-methylglucoside with acrylic acid and β-methylglucoside with methacrylic acid were 59.3% after 12 h and 71.3% after 72 h, respectively. The structural analysis of the products was performed by FT-IR spectroscopy and 1H NMR.

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