Enzymatic synthesis and biological characterization of a novel mangiferin glucoside

Iis Septiana; Thi Thanh Hanh Nguyen; Sangyong Lim; Seonmin Lee; Byeongsu Park; Sohyung Kwak; Sunghee Park; Seong Bo Kim; Doman Kim

doi:10.1016/j.enzmictec.2019.109479

Enzymatic synthesis and biological characterization of a novel mangiferin glucoside

Iis Septiana, Thi Thanh Hanh Nguyen, Sangyong Lim, Seonmin Lee, Byeongsu Park, Sohyung Kwak, Sunghee Park, Seong Bo Kim, Doman Kim

Global Leadership Division

Research output: Contribution to journal › Article › peer-review

Abstract

Mangiferin, a major constituent of Mangifera indica L., has attracted substantial attention due to its anti-oxidant, anti-diabetic, anti-inflammatory, and anti-microbial activities. However, its poor solubility in water limits its use in food and pharmaceutical industries. In this study, novel mangiferin-(1→6)-α-D-glucopyranoside (Mg-G1) was enzymatically synthesized from mangiferin and sucrose using glucansucrase from Leuconostoc mesenteroides B-512F/KM, and optimized using response surface methodology. The water solubility of Mg-G1 was found to be 824.7 mM, which is more than 2300-fold higher than that of mangiferin. Mg-G1 also showed DPPH radical scavenging activity and superoxide dismutase (SOD)-like scavenging activity, which were 4.77- and 3.71-fold higher than that of mangiferin, respectively. Mg-G1 displayed inhibitory activity against human intestinal maltase and COX-2. Thus, the novel glucosylated mangiferin may be used as an ingredient in functional food and pharmaceutical application.

Original language	English
Article number	109479
Journal	Enzyme and Microbial Technology
Volume	134
DOIs	https://doi.org/10.1016/j.enzmictec.2019.109479
Publication status	Published - 2020 Mar

Bibliographical note

Funding Information:
This research was partially supported by the Nuclear R&D program of Ministry of Science and ICT (MSIT), Republic of Korea , and by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (IPET) through the Agriculture, Food and Rural Affairs Research Center Support Program, funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) (D. Kim, 710012-03-1-HD220 ), and by the IPET through the High Value-added Food Technology Development Program ( 116013032HD020 ) funded by the MAFRA, Republic of Korea . The present study has been also conducted by the research grants ( 2018R1D1A1A09083366 , D. Kim, 2018R1D1A1B07049569 , T.T.H. Nguyen) of NRF, Republic of Korea and by the OTTOGI Corporation through the Research and Publication Project. Appendix A

Funding Information:
This research was partially supported by the Nuclear R&D program of Ministry of Science and ICT (MSIT), Republic of Korea, and by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (IPET) through the Agriculture, Food and Rural Affairs Research Center Support Program, funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) (D. Kim, 710012-03-1-HD220), and by the IPET through the High Value-added Food Technology Development Program (116013032HD020) funded by the MAFRA, Republic of Korea. The present study has been also conducted by the research grants (2018R1D1A1A09083366, D. Kim, 2018R1D1A1B07049569, T.T.H. Nguyen) of NRF, Republic of Korea and by the OTTOGI Corporation through the Research and Publication Project.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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@article{7b25e65d52ab4b7097bae9beccda868b,

title = "Enzymatic synthesis and biological characterization of a novel mangiferin glucoside",

abstract = "Mangiferin, a major constituent of Mangifera indica L., has attracted substantial attention due to its anti-oxidant, anti-diabetic, anti-inflammatory, and anti-microbial activities. However, its poor solubility in water limits its use in food and pharmaceutical industries. In this study, novel mangiferin-(1→6)-α-D-glucopyranoside (Mg-G1) was enzymatically synthesized from mangiferin and sucrose using glucansucrase from Leuconostoc mesenteroides B-512F/KM, and optimized using response surface methodology. The water solubility of Mg-G1 was found to be 824.7 mM, which is more than 2300-fold higher than that of mangiferin. Mg-G1 also showed DPPH radical scavenging activity and superoxide dismutase (SOD)-like scavenging activity, which were 4.77- and 3.71-fold higher than that of mangiferin, respectively. Mg-G1 displayed inhibitory activity against human intestinal maltase and COX-2. Thus, the novel glucosylated mangiferin may be used as an ingredient in functional food and pharmaceutical application.",

author = "Iis Septiana and Nguyen, {Thi Thanh Hanh} and Sangyong Lim and Seonmin Lee and Byeongsu Park and Sohyung Kwak and Sunghee Park and Kim, {Seong Bo} and Doman Kim",

note = "Funding Information: This research was partially supported by the Nuclear R&D program of Ministry of Science and ICT (MSIT), Republic of Korea , and by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (IPET) through the Agriculture, Food and Rural Affairs Research Center Support Program, funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) (D. Kim, 710012-03-1-HD220 ), and by the IPET through the High Value-added Food Technology Development Program ( 116013032HD020 ) funded by the MAFRA, Republic of Korea . The present study has been also conducted by the research grants ( 2018R1D1A1A09083366 , D. Kim, 2018R1D1A1B07049569 , T.T.H. Nguyen) of NRF, Republic of Korea and by the OTTOGI Corporation through the Research and Publication Project. Appendix A Funding Information: This research was partially supported by the Nuclear R&D program of Ministry of Science and ICT (MSIT), Republic of Korea, and by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (IPET) through the Agriculture, Food and Rural Affairs Research Center Support Program, funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) (D. Kim, 710012-03-1-HD220), and by the IPET through the High Value-added Food Technology Development Program (116013032HD020) funded by the MAFRA, Republic of Korea. The present study has been also conducted by the research grants (2018R1D1A1A09083366, D. Kim, 2018R1D1A1B07049569, T.T.H. Nguyen) of NRF, Republic of Korea and by the OTTOGI Corporation through the Research and Publication Project.",

year = "2020",

month = mar,

doi = "10.1016/j.enzmictec.2019.109479",

language = "English",

volume = "134",

journal = "Enzyme and Microbial Technology",

issn = "0141-0229",

publisher = "Elsevier Inc.",

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T1 - Enzymatic synthesis and biological characterization of a novel mangiferin glucoside

AU - Septiana, Iis

AU - Nguyen, Thi Thanh Hanh

AU - Lim, Sangyong

AU - Lee, Seonmin

AU - Park, Byeongsu

AU - Kwak, Sohyung

AU - Park, Sunghee

AU - Kim, Seong Bo

AU - Kim, Doman

N1 - Funding Information: This research was partially supported by the Nuclear R&D program of Ministry of Science and ICT (MSIT), Republic of Korea , and by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (IPET) through the Agriculture, Food and Rural Affairs Research Center Support Program, funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) (D. Kim, 710012-03-1-HD220 ), and by the IPET through the High Value-added Food Technology Development Program ( 116013032HD020 ) funded by the MAFRA, Republic of Korea . The present study has been also conducted by the research grants ( 2018R1D1A1A09083366 , D. Kim, 2018R1D1A1B07049569 , T.T.H. Nguyen) of NRF, Republic of Korea and by the OTTOGI Corporation through the Research and Publication Project. Appendix A Funding Information: This research was partially supported by the Nuclear R&D program of Ministry of Science and ICT (MSIT), Republic of Korea, and by the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry, and Fisheries (IPET) through the Agriculture, Food and Rural Affairs Research Center Support Program, funded by the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) (D. Kim, 710012-03-1-HD220), and by the IPET through the High Value-added Food Technology Development Program (116013032HD020) funded by the MAFRA, Republic of Korea. The present study has been also conducted by the research grants (2018R1D1A1A09083366, D. Kim, 2018R1D1A1B07049569, T.T.H. Nguyen) of NRF, Republic of Korea and by the OTTOGI Corporation through the Research and Publication Project.

PY - 2020/3

Y1 - 2020/3

N2 - Mangiferin, a major constituent of Mangifera indica L., has attracted substantial attention due to its anti-oxidant, anti-diabetic, anti-inflammatory, and anti-microbial activities. However, its poor solubility in water limits its use in food and pharmaceutical industries. In this study, novel mangiferin-(1→6)-α-D-glucopyranoside (Mg-G1) was enzymatically synthesized from mangiferin and sucrose using glucansucrase from Leuconostoc mesenteroides B-512F/KM, and optimized using response surface methodology. The water solubility of Mg-G1 was found to be 824.7 mM, which is more than 2300-fold higher than that of mangiferin. Mg-G1 also showed DPPH radical scavenging activity and superoxide dismutase (SOD)-like scavenging activity, which were 4.77- and 3.71-fold higher than that of mangiferin, respectively. Mg-G1 displayed inhibitory activity against human intestinal maltase and COX-2. Thus, the novel glucosylated mangiferin may be used as an ingredient in functional food and pharmaceutical application.

AB - Mangiferin, a major constituent of Mangifera indica L., has attracted substantial attention due to its anti-oxidant, anti-diabetic, anti-inflammatory, and anti-microbial activities. However, its poor solubility in water limits its use in food and pharmaceutical industries. In this study, novel mangiferin-(1→6)-α-D-glucopyranoside (Mg-G1) was enzymatically synthesized from mangiferin and sucrose using glucansucrase from Leuconostoc mesenteroides B-512F/KM, and optimized using response surface methodology. The water solubility of Mg-G1 was found to be 824.7 mM, which is more than 2300-fold higher than that of mangiferin. Mg-G1 also showed DPPH radical scavenging activity and superoxide dismutase (SOD)-like scavenging activity, which were 4.77- and 3.71-fold higher than that of mangiferin, respectively. Mg-G1 displayed inhibitory activity against human intestinal maltase and COX-2. Thus, the novel glucosylated mangiferin may be used as an ingredient in functional food and pharmaceutical application.

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