Enzymatic synthesis and biological characterization of a novel mangiferin glucoside

Iis Septiana, Thi Thanh Hanh Nguyen, Sangyong Lim, Seonmin Lee, Byeongsu Park, Sohyung Kwak, Sunghee Park, Seong Bo Kim, Doman Kim

Research output: Contribution to journalArticle


Mangiferin, a major constituent of Mangifera indica L., has attracted substantial attention due to its anti-oxidant, anti-diabetic, anti-inflammatory, and anti-microbial activities. However, its poor solubility in water limits its use in food and pharmaceutical industries. In this study, novel mangiferin-(1→6)-α-D-glucopyranoside (Mg-G1) was enzymatically synthesized from mangiferin and sucrose using glucansucrase from Leuconostoc mesenteroides B-512F/KM, and optimized using response surface methodology. The water solubility of Mg-G1 was found to be 824.7 mM, which is more than 2300-fold higher than that of mangiferin. Mg-G1 also showed DPPH radical scavenging activity and superoxide dismutase (SOD)-like scavenging activity, which were 4.77- and 3.71-fold higher than that of mangiferin, respectively. Mg-G1 displayed inhibitory activity against human intestinal maltase and COX-2. Thus, the novel glucosylated mangiferin may be used as an ingredient in functional food and pharmaceutical application.

Original languageEnglish
Article number109479
JournalEnzyme and Microbial Technology
Publication statusPublished - 2020 Mar

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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    Septiana, I., Nguyen, T. T. H., Lim, S., Lee, S., Park, B., Kwak, S., Park, S., Kim, S. B., & Kim, D. (2020). Enzymatic synthesis and biological characterization of a novel mangiferin glucoside. Enzyme and Microbial Technology, 134, [109479]. https://doi.org/10.1016/j.enzmictec.2019.109479