Enzymatic Synthesis and Properties of Trehalose Analogues as Disaccharide and Trisaccharide

Soo Bok Lee, Soo In Ryu, Hye Min Kim, Bong Gwan Kim

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Trehalose analogue, non-reducing dissacharide of 1α-D-glucopyranosyl α-D-galactopyranoside, was synthesized by Pyrococcus horikoshii glycosyltransferase transglycosylation reaction with sugar nucleotides and galactose. This disaccharide analogue was effective inhibitor for several disaccharidases including rat intestinal trehalase and sucrase. Trehalose was also modified by Escherichia coli β-galactosidase transglycosylation reaction with lactose to give trehalose trisaccharide analogues. These trisaccharide analogues have been supposed to be indigestible oligosaccharides exhibiting enhanced hygroscopic, cryoprotective, anti-cariogenic, and prebiotic effects. The enzymatic techniques using glycosyltransferase and glycosidase might lead to create more trehalose-based analogues with a wide variety of acceptor and donor sugars.

Original languageEnglish
Title of host publicationCarbohydrate-Active Enzymes
Subtitle of host publicationStructure, Function and Applications
PublisherElsevier Ltd
Pages258-265
Number of pages8
ISBN (Print)9781845695194
DOIs
Publication statusPublished - 2008 Sep

Bibliographical note

Funding Information:
We thank for financial support from the Marine and Extreme Genome Research Center Program, Ministry of Maritime Affairs and Fisheries, Republic of Korea.

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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