Enzyme activities

Hojeong Kang, Seon Young Kim, Chris Freeman

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Extracellular enzymes, originating primarily from microorganisms and the roots of plants, play a pivotal role in wetland biogeochemistry by driving organic matter transformations. For example, in wetlands, extracellular enzyme activities have been considered in relation to nutrient availability, efficiency of wastewater treatment, decomposition of soil organic matter, and trace gas emission. The effects of environmental conditions on enzyme activities have also drawn much attention. For example, the impacts of water level drawdown, elevated CO2, O3, ultraviolet radiation, temperature change, land use change, and heavy metals have all been addressed. In this chapter, a method for the measurement of extracellular enzyme activity through the use of fluorogenic model substrates is described. Methylumbelliferyl substrates are prepared and added to wetland soils, and the fluorescence produced is quantified and presented as enzyme activity. This method is highly sensitive, with high reproducibility, and hence represents a particularly effective measure of extracellular enzyme activities in wetlands.

Original languageEnglish
Title of host publicationMethods in Biogeochemistry of Wetlands
Publisherwiley
Pages373-384
Number of pages12
ISBN (Electronic)9780891189619
ISBN (Print)9780891189602
DOIs
Publication statusPublished - 2015 Jan 1

Bibliographical note

Publisher Copyright:
© 2013 by Soil Science Society of America.

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Agricultural and Biological Sciences(all)

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