Enzyme immobilization on metal oxide semiconductors exploiting amine functionalized layer

Hojoong Kim, Jang Yeon Kwon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The immobilization of glucose oxidase (GOx) on indium-gallium zinc oxide (IGZO) thin films is studied in order to fabricate a high performance biosensor. An amine functionalized layer, using (3-aminopropyl)triethoxysilane (APTES), is employed to support the immobilization and to achieve a qualified silanization layer. Fourier Transform Infrared (FT-IR) Spectroscopy, Atomic Force Microscopy (AFM), and contact angle measurements were performed to analyze the surface characteristics and to investigate a deposition mechanism for APTES with varying concentrations. Finally, GOx is immobilized on the APTES layer to determine how a diverse surface quality influences GOx density, and its enzymatic activity is identified by the detection of the electrical signal from glucose biosensors.

Original languageEnglish
Pages (from-to)19656-19661
Number of pages6
JournalRSC Advances
Volume7
Issue number32
DOIs
Publication statusPublished - 2017

Bibliographical note

Funding Information:
This research was supported by the MSIP (Ministry of Science, ICT and Future Planning), Korea, under the ?ICT Consilience Creative Program? (IITP-R0346-16-1008) supervised by the IITP (Institute for Information and communications Technology Promotion).

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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