Enzyme immobilization on metal oxide semiconductors exploiting amine functionalized layer

Hojoong Kim, Jang Yeon Kwon

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The immobilization of glucose oxidase (GOx) on indium-gallium zinc oxide (IGZO) thin films is studied in order to fabricate a high performance biosensor. An amine functionalized layer, using (3-aminopropyl)triethoxysilane (APTES), is employed to support the immobilization and to achieve a qualified silanization layer. Fourier Transform Infrared (FT-IR) Spectroscopy, Atomic Force Microscopy (AFM), and contact angle measurements were performed to analyze the surface characteristics and to investigate a deposition mechanism for APTES with varying concentrations. Finally, GOx is immobilized on the APTES layer to determine how a diverse surface quality influences GOx density, and its enzymatic activity is identified by the detection of the electrical signal from glucose biosensors.

Original languageEnglish
Pages (from-to)19656-19661
Number of pages6
JournalRSC Advances
Volume7
Issue number32
DOIs
Publication statusPublished - 2017 Jan 1

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Enzyme immobilization
Glucose Oxidase
Glucose oxidase
Amines
Metals
Biosensors
Zinc Oxide
Gallium
Indium
Angle measurement
Zinc oxide
Oxide films
Contact angle
Surface properties
Fourier transform infrared spectroscopy
Glucose
Atomic force microscopy
Thin films
amino-propyl-triethoxysilane
Oxide semiconductors

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

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abstract = "The immobilization of glucose oxidase (GOx) on indium-gallium zinc oxide (IGZO) thin films is studied in order to fabricate a high performance biosensor. An amine functionalized layer, using (3-aminopropyl)triethoxysilane (APTES), is employed to support the immobilization and to achieve a qualified silanization layer. Fourier Transform Infrared (FT-IR) Spectroscopy, Atomic Force Microscopy (AFM), and contact angle measurements were performed to analyze the surface characteristics and to investigate a deposition mechanism for APTES with varying concentrations. Finally, GOx is immobilized on the APTES layer to determine how a diverse surface quality influences GOx density, and its enzymatic activity is identified by the detection of the electrical signal from glucose biosensors.",
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Enzyme immobilization on metal oxide semiconductors exploiting amine functionalized layer. / Kim, Hojoong; Kwon, Jang Yeon.

In: RSC Advances, Vol. 7, No. 32, 01.01.2017, p. 19656-19661.

Research output: Contribution to journalArticle

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AU - Kwon, Jang Yeon

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