Eop1 from a rubus strain of erwinia amylovora functions as a host-range limiting factor

J. E. Asselin, J. M. Bonasera, Jihyun F. Kim, C. S. Oh, S. V. Beer

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Strains of Erwinia amylovora, the bacterium causing the disease fire blight of rosaceous plants, are separated into two groups based on host range: Spiraeoideae and Rubus strains. Spiraeoideae strains have wide host ranges, infecting plants in many rosaceous genera, including apple and pear. In the field, Rubus strains infect the genus Rubus exclusively, which includes raspberry and blackberry. Based on comparisons of limited sequence data from a Rubus and a Spiraeoideae strain, the gene eop1 was identified as unusually divergent, and it was selected as a possible host specificity factor. To test this, eop1 genes from a Rubus strain and a Spiraeoideae strain were cloned and mutated. Expression of the Rubus-strain eop1 reduced the virulence of E. amylovora in immature pear fruit and in apple shoots. Sequencing the orfA-eop1 regions of several strains of E. amylovora confirmed that forms of eop1 are conserved among strains with similar host ranges. This work provides evidence that eop1 from a Rubus-specific strain can function as a determinant of host specificity in E. amylovora.

Original languageEnglish
Pages (from-to)935-944
Number of pages10
JournalPhytopathology
Volume101
Issue number8
DOIs
Publication statusPublished - 2011 Aug 1

Fingerprint

Rubus
Erwinia amylovora
host range
host specificity
pears
apples
blackberries
raspberries
virulence
genes
immatures

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Asselin, J. E. ; Bonasera, J. M. ; Kim, Jihyun F. ; Oh, C. S. ; Beer, S. V. / Eop1 from a rubus strain of erwinia amylovora functions as a host-range limiting factor. In: Phytopathology. 2011 ; Vol. 101, No. 8. pp. 935-944.
@article{114de7f432324efebec2a75ea4ddb96e,
title = "Eop1 from a rubus strain of erwinia amylovora functions as a host-range limiting factor",
abstract = "Strains of Erwinia amylovora, the bacterium causing the disease fire blight of rosaceous plants, are separated into two groups based on host range: Spiraeoideae and Rubus strains. Spiraeoideae strains have wide host ranges, infecting plants in many rosaceous genera, including apple and pear. In the field, Rubus strains infect the genus Rubus exclusively, which includes raspberry and blackberry. Based on comparisons of limited sequence data from a Rubus and a Spiraeoideae strain, the gene eop1 was identified as unusually divergent, and it was selected as a possible host specificity factor. To test this, eop1 genes from a Rubus strain and a Spiraeoideae strain were cloned and mutated. Expression of the Rubus-strain eop1 reduced the virulence of E. amylovora in immature pear fruit and in apple shoots. Sequencing the orfA-eop1 regions of several strains of E. amylovora confirmed that forms of eop1 are conserved among strains with similar host ranges. This work provides evidence that eop1 from a Rubus-specific strain can function as a determinant of host specificity in E. amylovora.",
author = "Asselin, {J. E.} and Bonasera, {J. M.} and Kim, {Jihyun F.} and Oh, {C. S.} and Beer, {S. V.}",
year = "2011",
month = "8",
day = "1",
doi = "10.1094/PHYTO-12-10-0339",
language = "English",
volume = "101",
pages = "935--944",
journal = "Phytopathology",
issn = "0031-949X",
publisher = "American Phytopathological Society",
number = "8",

}

Eop1 from a rubus strain of erwinia amylovora functions as a host-range limiting factor. / Asselin, J. E.; Bonasera, J. M.; Kim, Jihyun F.; Oh, C. S.; Beer, S. V.

In: Phytopathology, Vol. 101, No. 8, 01.08.2011, p. 935-944.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Eop1 from a rubus strain of erwinia amylovora functions as a host-range limiting factor

AU - Asselin, J. E.

AU - Bonasera, J. M.

AU - Kim, Jihyun F.

AU - Oh, C. S.

AU - Beer, S. V.

PY - 2011/8/1

Y1 - 2011/8/1

N2 - Strains of Erwinia amylovora, the bacterium causing the disease fire blight of rosaceous plants, are separated into two groups based on host range: Spiraeoideae and Rubus strains. Spiraeoideae strains have wide host ranges, infecting plants in many rosaceous genera, including apple and pear. In the field, Rubus strains infect the genus Rubus exclusively, which includes raspberry and blackberry. Based on comparisons of limited sequence data from a Rubus and a Spiraeoideae strain, the gene eop1 was identified as unusually divergent, and it was selected as a possible host specificity factor. To test this, eop1 genes from a Rubus strain and a Spiraeoideae strain were cloned and mutated. Expression of the Rubus-strain eop1 reduced the virulence of E. amylovora in immature pear fruit and in apple shoots. Sequencing the orfA-eop1 regions of several strains of E. amylovora confirmed that forms of eop1 are conserved among strains with similar host ranges. This work provides evidence that eop1 from a Rubus-specific strain can function as a determinant of host specificity in E. amylovora.

AB - Strains of Erwinia amylovora, the bacterium causing the disease fire blight of rosaceous plants, are separated into two groups based on host range: Spiraeoideae and Rubus strains. Spiraeoideae strains have wide host ranges, infecting plants in many rosaceous genera, including apple and pear. In the field, Rubus strains infect the genus Rubus exclusively, which includes raspberry and blackberry. Based on comparisons of limited sequence data from a Rubus and a Spiraeoideae strain, the gene eop1 was identified as unusually divergent, and it was selected as a possible host specificity factor. To test this, eop1 genes from a Rubus strain and a Spiraeoideae strain were cloned and mutated. Expression of the Rubus-strain eop1 reduced the virulence of E. amylovora in immature pear fruit and in apple shoots. Sequencing the orfA-eop1 regions of several strains of E. amylovora confirmed that forms of eop1 are conserved among strains with similar host ranges. This work provides evidence that eop1 from a Rubus-specific strain can function as a determinant of host specificity in E. amylovora.

UR - http://www.scopus.com/inward/record.url?scp=80051702505&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051702505&partnerID=8YFLogxK

U2 - 10.1094/PHYTO-12-10-0339

DO - 10.1094/PHYTO-12-10-0339

M3 - Article

VL - 101

SP - 935

EP - 944

JO - Phytopathology

JF - Phytopathology

SN - 0031-949X

IS - 8

ER -