EPetri platform for continuous on-chip monitoring of microorganisms

Seung Ah Lee, Guoan Zheng, Nandini Mukherjee, Changhuei Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present ePetri, a self-imaging culture platform for continuous and long-term monitoring of microorganisms. The ePetri achieves on-chip microscopy by using complimentary metal-oxide semiconductor (CMOS) image sensors as a culture substrate. For motile microorganisms, we can perform pixel super-resolution image reconstruction by using the inherent motion of the cells, allowing high-resolution imaging without any optical element. We also demonstrate longitudinal imaging of Euglena gracilis cultured in ePetri with various image-based analysis for culture monitoring. As a miniaturized and automated culture monitoring platform, this ePetri technology can greatly improve studies and experiments.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages737-739
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012 Jan 1
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 2012 Oct 282012 Nov 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

Fingerprint

Microorganisms
Imaging techniques
Monitoring
Optical devices
Image reconstruction
Image sensors
Microscopic examination
Pixels
Metals
Substrates
Experiments
Oxide semiconductors

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Lee, S. A., Zheng, G., Mukherjee, N., & Yang, C. (2012). EPetri platform for continuous on-chip monitoring of microorganisms. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 737-739). Chemical and Biological Microsystems Society.
Lee, Seung Ah ; Zheng, Guoan ; Mukherjee, Nandini ; Yang, Changhuei. / EPetri platform for continuous on-chip monitoring of microorganisms. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 737-739
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abstract = "We present ePetri, a self-imaging culture platform for continuous and long-term monitoring of microorganisms. The ePetri achieves on-chip microscopy by using complimentary metal-oxide semiconductor (CMOS) image sensors as a culture substrate. For motile microorganisms, we can perform pixel super-resolution image reconstruction by using the inherent motion of the cells, allowing high-resolution imaging without any optical element. We also demonstrate longitudinal imaging of Euglena gracilis cultured in ePetri with various image-based analysis for culture monitoring. As a miniaturized and automated culture monitoring platform, this ePetri technology can greatly improve studies and experiments.",
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Lee, SA, Zheng, G, Mukherjee, N & Yang, C 2012, EPetri platform for continuous on-chip monitoring of microorganisms. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 737-739, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 12/10/28.

EPetri platform for continuous on-chip monitoring of microorganisms. / Lee, Seung Ah; Zheng, Guoan; Mukherjee, Nandini; Yang, Changhuei.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 737-739.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - We present ePetri, a self-imaging culture platform for continuous and long-term monitoring of microorganisms. The ePetri achieves on-chip microscopy by using complimentary metal-oxide semiconductor (CMOS) image sensors as a culture substrate. For motile microorganisms, we can perform pixel super-resolution image reconstruction by using the inherent motion of the cells, allowing high-resolution imaging without any optical element. We also demonstrate longitudinal imaging of Euglena gracilis cultured in ePetri with various image-based analysis for culture monitoring. As a miniaturized and automated culture monitoring platform, this ePetri technology can greatly improve studies and experiments.

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Lee SA, Zheng G, Mukherjee N, Yang C. EPetri platform for continuous on-chip monitoring of microorganisms. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 737-739