Epidemiological characteristics and molecular basis of fluoroquinolone-resistant Neisseria gonorrhoeae strains isolated in Korea and nearby countries

Dongeun Yong, Tae Sook Kim, Jong Rak Choi, Jong Hwa Yum, Kyungwon Lee, Yunsop Chong, Hee Bok Oh, Tiffany Shultz, John W. Tapsall

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objectives: This study was performed to examine the cause of the increase in quinolone-resistant Neisseria gonorrhoeae (QRNG) observed in Korea. Methods: The antimicrobial susceptibilities of 190 isolates of gonococci from Korea in 2000 were examined by NCCLS methods, and subsets of these isolates underwent mutation analysis of the quinolone resistance-determining regions (QRDRs) of gyrA and parC. Molecular epidemiological characterization of 25 Korean isolates and 54 isolates from overseas was performed by pulsed-field gel electrophoresis (PFGE) and the results compared. Results: Most (172, 90.5%) of the 190 gonococci tested displayed reduced susceptibility to ciprofloxacin. All strains with high-level ciprofloxacin resistance (ciprofloxacin MIC ≥ 4 mg/L) contained a double amino acid alteration at the 91 and 95 positions in the QRDR of GyrA and a single alteration in ParC. PFGE types of high-level QRNG in Korea were mostly different from those of other nearby countries. Conclusions: These results suggest that the observed increase in ciprofloxacin-resistant isolates is due to the mutation and spread of Korean multiclonal isolates rather than importation from overseas.

Original languageEnglish
Pages (from-to)451-455
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume54
Issue number2
DOIs
Publication statusPublished - 2004 Aug 1

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Neisseria gonorrhoeae
Quinolones
Fluoroquinolones
Korea
Ciprofloxacin
Pulsed Field Gel Electrophoresis
Mutation
Amino Acids

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Yong, Dongeun ; Kim, Tae Sook ; Choi, Jong Rak ; Yum, Jong Hwa ; Lee, Kyungwon ; Chong, Yunsop ; Oh, Hee Bok ; Shultz, Tiffany ; Tapsall, John W. / Epidemiological characteristics and molecular basis of fluoroquinolone-resistant Neisseria gonorrhoeae strains isolated in Korea and nearby countries. In: Journal of Antimicrobial Chemotherapy. 2004 ; Vol. 54, No. 2. pp. 451-455.
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Epidemiological characteristics and molecular basis of fluoroquinolone-resistant Neisseria gonorrhoeae strains isolated in Korea and nearby countries. / Yong, Dongeun; Kim, Tae Sook; Choi, Jong Rak; Yum, Jong Hwa; Lee, Kyungwon; Chong, Yunsop; Oh, Hee Bok; Shultz, Tiffany; Tapsall, John W.

In: Journal of Antimicrobial Chemotherapy, Vol. 54, No. 2, 01.08.2004, p. 451-455.

Research output: Contribution to journalArticle

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N2 - Objectives: This study was performed to examine the cause of the increase in quinolone-resistant Neisseria gonorrhoeae (QRNG) observed in Korea. Methods: The antimicrobial susceptibilities of 190 isolates of gonococci from Korea in 2000 were examined by NCCLS methods, and subsets of these isolates underwent mutation analysis of the quinolone resistance-determining regions (QRDRs) of gyrA and parC. Molecular epidemiological characterization of 25 Korean isolates and 54 isolates from overseas was performed by pulsed-field gel electrophoresis (PFGE) and the results compared. Results: Most (172, 90.5%) of the 190 gonococci tested displayed reduced susceptibility to ciprofloxacin. All strains with high-level ciprofloxacin resistance (ciprofloxacin MIC ≥ 4 mg/L) contained a double amino acid alteration at the 91 and 95 positions in the QRDR of GyrA and a single alteration in ParC. PFGE types of high-level QRNG in Korea were mostly different from those of other nearby countries. Conclusions: These results suggest that the observed increase in ciprofloxacin-resistant isolates is due to the mutation and spread of Korean multiclonal isolates rather than importation from overseas.

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