Epigallocatechin-3-gallate inhibits basic fibroblast growth factor-induced intracellular signaling transduction pathway in rat aortic smooth muscle cells

Ki Chul Hwang, Kyung Hye Lee, Yangsoo Jang, Yeo Pyo Yun, Kwang Hoe Chung

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Daily green tea drinking showed preventive effects on the progression of atherosclerosis. Although epigallocatechin-3-gallate [EGCG] has anti-proliferative effects on various cells, relatively little is known about the molecular mechanisms of the anti-proliferative effects of EGCG. To determine whether the transduction signals and protooncogene expression were affected by EGCG, this study investigated the molecular mechanism of the anti-proliferative effects in basic fibroblast growth factor (bFGF)-stimulated rat aortic smooth muscle cells (RAoSMCs). EGCG inhibited the proliferative response stimulated by 10% fetal bovine serum dose dependently in RAoSMCs (median inhibitory concentration [IC50]: 28.4 × 10-6 M). EGCG also inhibited the migration of bFGF-stimulated RAoSMCs in a dose-dependent manner, showing that 21.8 × 10-6 M of EGCG significantly inhibited the migration by 75 ± 5% in comparison with bFGF-stimulated migration. In RAoSMCs, EGCG dramatically inhibited Ras activation and c-jun N-terminal kinase (JNK) activity without affecting protein kinase C expression. Induction of c-jun mRNA stimulated by bFGF was significantly reduced dose dependently up to 87.3 × 10-6 M of EGCG. These results indicate that the anti-proliferative effect of EGCG on RAoSMCs is partly Ras/JNK mediated, independent of protein kinase C, and is attributable to the downregulation of c-jun expression.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalJournal of Cardiovascular Pharmacology
Volume39
Issue number2
DOIs
Publication statusPublished - 2002 Feb 5

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Fibroblast Growth Factor 2
Smooth Muscle Myocytes
JNK Mitogen-Activated Protein Kinases
Protein Kinase C
epigallocatechin gallate
Tea
Inhibitory Concentration 50
Drinking
Signal Transduction
Atherosclerosis
Down-Regulation
Messenger RNA
Serum

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

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title = "Epigallocatechin-3-gallate inhibits basic fibroblast growth factor-induced intracellular signaling transduction pathway in rat aortic smooth muscle cells",
abstract = "Daily green tea drinking showed preventive effects on the progression of atherosclerosis. Although epigallocatechin-3-gallate [EGCG] has anti-proliferative effects on various cells, relatively little is known about the molecular mechanisms of the anti-proliferative effects of EGCG. To determine whether the transduction signals and protooncogene expression were affected by EGCG, this study investigated the molecular mechanism of the anti-proliferative effects in basic fibroblast growth factor (bFGF)-stimulated rat aortic smooth muscle cells (RAoSMCs). EGCG inhibited the proliferative response stimulated by 10{\%} fetal bovine serum dose dependently in RAoSMCs (median inhibitory concentration [IC50]: 28.4 × 10-6 M). EGCG also inhibited the migration of bFGF-stimulated RAoSMCs in a dose-dependent manner, showing that 21.8 × 10-6 M of EGCG significantly inhibited the migration by 75 ± 5{\%} in comparison with bFGF-stimulated migration. In RAoSMCs, EGCG dramatically inhibited Ras activation and c-jun N-terminal kinase (JNK) activity without affecting protein kinase C expression. Induction of c-jun mRNA stimulated by bFGF was significantly reduced dose dependently up to 87.3 × 10-6 M of EGCG. These results indicate that the anti-proliferative effect of EGCG on RAoSMCs is partly Ras/JNK mediated, independent of protein kinase C, and is attributable to the downregulation of c-jun expression.",
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Epigallocatechin-3-gallate inhibits basic fibroblast growth factor-induced intracellular signaling transduction pathway in rat aortic smooth muscle cells. / Hwang, Ki Chul; Lee, Kyung Hye; Jang, Yangsoo; Yun, Yeo Pyo; Chung, Kwang Hoe.

In: Journal of Cardiovascular Pharmacology, Vol. 39, No. 2, 05.02.2002, p. 271-277.

Research output: Contribution to journalArticle

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AU - Chung, Kwang Hoe

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