Epigallocatechin-3-O -gallate-loaded poly(lactic-co-glycolic acid) fibrous sheets as anti-adhesion barriers

Jong Ho Lee, Yong Cheol Shin, Won Jun Yang, Jongchul Park, Suong Hyu Hyon, Dong Wook Han

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Epigallocatechin-3-O -gallate (EGCG), the main polyphenolic component of green tea, has a wide range of pharmacological activities, including antioxidant, anti-inflammatory, and anti-fibrotic effects. In this study, EGCG-loaded poly(lactic-coglycolic acid) (PLGA) sheets were prepared by electrospinning nanofibers and evaluating their potential as tissue-adhesion barriers. EGCG-loaded PLGA (E-PLGA) fibrous sheets were electrospun from a PLGA solution containing 8% (w/v) EGCG. The average diameter of E-PLGA fibers was 397 ± 159 nm, which was comparable to that of pure PLGA fibers (459 ± 154 nm). EGCG was uniformly dispersed in E-PLGA sheets without direct chemical interactions. E-PLGA fibrous sheets showed sustained release of EGCG by controlled diffusion and PLGA degradation. The attachment and proliferation of L-929 fibroblastic cells were significantly (p < 0.05) suppressed in E-PLGA sheets. Furthermore, E-PLGA fibrous sheets did not induce any inflammatory response to J774A.1 macrophages. The anti-adhesion efficacy of E-PLGA fibrous sheets was evaluated in the intraperitoneal adhesion model in rats. Two weeks after surgical treatment, macroscopic adhesion (extent and severity) scores and histopathological tissue responses of E-PLGA fibrous sheets were significantly lower than those of non-treated controls and pure PLGA sheets. The results suggest that the scores are comparable, and in some cases superior, to those of other commercialized tissue-adhesion barriers. In conclusion, our study findings suggest that E-PLGA fibrous sheets may be exploited as potential tissue-adhesion barriers for the prevention of post-surgical adhesion formation.

Original languageEnglish
Pages (from-to)1461-1471
Number of pages11
JournalJournal of Biomedical Nanotechnology
Volume11
Issue number8
DOIs
Publication statusPublished - 2014 Aug 1

Fingerprint

Adhesion
Acids
Tissue Adhesions
Tissue
Fibers
Macrophages
Electrospinning
Lactic acid
Nanofibers
Milk
polylactic acid-polyglycolic acid copolymer
epigallocatechin gallate
Antioxidants
Rats
Degradation
Tea
Anti-Inflammatory Agents
Pharmacology

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Lee, Jong Ho ; Shin, Yong Cheol ; Yang, Won Jun ; Park, Jongchul ; Hyon, Suong Hyu ; Han, Dong Wook. / Epigallocatechin-3-O -gallate-loaded poly(lactic-co-glycolic acid) fibrous sheets as anti-adhesion barriers. In: Journal of Biomedical Nanotechnology. 2014 ; Vol. 11, No. 8. pp. 1461-1471.
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abstract = "Epigallocatechin-3-O -gallate (EGCG), the main polyphenolic component of green tea, has a wide range of pharmacological activities, including antioxidant, anti-inflammatory, and anti-fibrotic effects. In this study, EGCG-loaded poly(lactic-coglycolic acid) (PLGA) sheets were prepared by electrospinning nanofibers and evaluating their potential as tissue-adhesion barriers. EGCG-loaded PLGA (E-PLGA) fibrous sheets were electrospun from a PLGA solution containing 8{\%} (w/v) EGCG. The average diameter of E-PLGA fibers was 397 ± 159 nm, which was comparable to that of pure PLGA fibers (459 ± 154 nm). EGCG was uniformly dispersed in E-PLGA sheets without direct chemical interactions. E-PLGA fibrous sheets showed sustained release of EGCG by controlled diffusion and PLGA degradation. The attachment and proliferation of L-929 fibroblastic cells were significantly (p < 0.05) suppressed in E-PLGA sheets. Furthermore, E-PLGA fibrous sheets did not induce any inflammatory response to J774A.1 macrophages. The anti-adhesion efficacy of E-PLGA fibrous sheets was evaluated in the intraperitoneal adhesion model in rats. Two weeks after surgical treatment, macroscopic adhesion (extent and severity) scores and histopathological tissue responses of E-PLGA fibrous sheets were significantly lower than those of non-treated controls and pure PLGA sheets. The results suggest that the scores are comparable, and in some cases superior, to those of other commercialized tissue-adhesion barriers. In conclusion, our study findings suggest that E-PLGA fibrous sheets may be exploited as potential tissue-adhesion barriers for the prevention of post-surgical adhesion formation.",
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Epigallocatechin-3-O -gallate-loaded poly(lactic-co-glycolic acid) fibrous sheets as anti-adhesion barriers. / Lee, Jong Ho; Shin, Yong Cheol; Yang, Won Jun; Park, Jongchul; Hyon, Suong Hyu; Han, Dong Wook.

In: Journal of Biomedical Nanotechnology, Vol. 11, No. 8, 01.08.2014, p. 1461-1471.

Research output: Contribution to journalArticle

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T1 - Epigallocatechin-3-O -gallate-loaded poly(lactic-co-glycolic acid) fibrous sheets as anti-adhesion barriers

AU - Lee, Jong Ho

AU - Shin, Yong Cheol

AU - Yang, Won Jun

AU - Park, Jongchul

AU - Hyon, Suong Hyu

AU - Han, Dong Wook

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AB - Epigallocatechin-3-O -gallate (EGCG), the main polyphenolic component of green tea, has a wide range of pharmacological activities, including antioxidant, anti-inflammatory, and anti-fibrotic effects. In this study, EGCG-loaded poly(lactic-coglycolic acid) (PLGA) sheets were prepared by electrospinning nanofibers and evaluating their potential as tissue-adhesion barriers. EGCG-loaded PLGA (E-PLGA) fibrous sheets were electrospun from a PLGA solution containing 8% (w/v) EGCG. The average diameter of E-PLGA fibers was 397 ± 159 nm, which was comparable to that of pure PLGA fibers (459 ± 154 nm). EGCG was uniformly dispersed in E-PLGA sheets without direct chemical interactions. E-PLGA fibrous sheets showed sustained release of EGCG by controlled diffusion and PLGA degradation. The attachment and proliferation of L-929 fibroblastic cells were significantly (p < 0.05) suppressed in E-PLGA sheets. Furthermore, E-PLGA fibrous sheets did not induce any inflammatory response to J774A.1 macrophages. The anti-adhesion efficacy of E-PLGA fibrous sheets was evaluated in the intraperitoneal adhesion model in rats. Two weeks after surgical treatment, macroscopic adhesion (extent and severity) scores and histopathological tissue responses of E-PLGA fibrous sheets were significantly lower than those of non-treated controls and pure PLGA sheets. The results suggest that the scores are comparable, and in some cases superior, to those of other commercialized tissue-adhesion barriers. In conclusion, our study findings suggest that E-PLGA fibrous sheets may be exploited as potential tissue-adhesion barriers for the prevention of post-surgical adhesion formation.

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