Abstract
The dissemination of circulating tumor cells (CTCs) requires the Epithelial-to-Mesenchymal transition (EMT), in which cells lose their epithelial characteristics and acquire more mesenchymal-like phenotypes. Current isolation of CTCs relies on affinity-based approaches reliant on the expression of Epithelial Cell Adhesion Molecule (EpCAM). Here we show EMT-induced breast cancer cells maintained in prolonged mammosphere culture conditions possess increased EMT markers and cancer stem cell markers, as well as reduced cell mass and size by quantitative phase microscopy; however, EpCAM expression is dramatically decreased in these cells. Moreover, CTCs isolated from breast cancer patients using a label-free microfluidic flow fractionation device had differing expression patterns of EpCAM, indicating that affinity approaches reliant on EpCAM expression may underestimate CTC number and potentially miss critical subpopulations. Further characterization of CTCs, including low-EpCAM populations, using this technology may improve detection techniques and cancer diagnosis, ultimately improving cancer treatment.
| Original language | English |
|---|---|
| Pages (from-to) | 24677-24687 |
| Number of pages | 11 |
| Journal | Oncotarget |
| Volume | 7 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 2016 Apr 26 |
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All Science Journal Classification (ASJC) codes
- Oncology
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Epithelial-to-mesenchymal transition leads to loss of EpCAM and different physical properties in circulating tumor cells from metastatic breast cancer. / Kyung-A Hyun, Hyun; Goo, Ki Bang; Han, Hyunju; Sohn, Joohyuk; Choi, Wonshik; Kim, Seung Il; Jung, Hyo il; Kim, You Sun.
In: Oncotarget, Vol. 7, No. 17, 26.04.2016, p. 24677-24687.Research output: Contribution to journal › Article
TY - JOUR
T1 - Epithelial-to-mesenchymal transition leads to loss of EpCAM and different physical properties in circulating tumor cells from metastatic breast cancer
AU - Kyung-A Hyun, Hyun
AU - Goo, Ki Bang
AU - Han, Hyunju
AU - Sohn, Joohyuk
AU - Choi, Wonshik
AU - Kim, Seung Il
AU - Jung, Hyo il
AU - Kim, You Sun
PY - 2016/4/26
Y1 - 2016/4/26
N2 - The dissemination of circulating tumor cells (CTCs) requires the Epithelial-to-Mesenchymal transition (EMT), in which cells lose their epithelial characteristics and acquire more mesenchymal-like phenotypes. Current isolation of CTCs relies on affinity-based approaches reliant on the expression of Epithelial Cell Adhesion Molecule (EpCAM). Here we show EMT-induced breast cancer cells maintained in prolonged mammosphere culture conditions possess increased EMT markers and cancer stem cell markers, as well as reduced cell mass and size by quantitative phase microscopy; however, EpCAM expression is dramatically decreased in these cells. Moreover, CTCs isolated from breast cancer patients using a label-free microfluidic flow fractionation device had differing expression patterns of EpCAM, indicating that affinity approaches reliant on EpCAM expression may underestimate CTC number and potentially miss critical subpopulations. Further characterization of CTCs, including low-EpCAM populations, using this technology may improve detection techniques and cancer diagnosis, ultimately improving cancer treatment.
AB - The dissemination of circulating tumor cells (CTCs) requires the Epithelial-to-Mesenchymal transition (EMT), in which cells lose their epithelial characteristics and acquire more mesenchymal-like phenotypes. Current isolation of CTCs relies on affinity-based approaches reliant on the expression of Epithelial Cell Adhesion Molecule (EpCAM). Here we show EMT-induced breast cancer cells maintained in prolonged mammosphere culture conditions possess increased EMT markers and cancer stem cell markers, as well as reduced cell mass and size by quantitative phase microscopy; however, EpCAM expression is dramatically decreased in these cells. Moreover, CTCs isolated from breast cancer patients using a label-free microfluidic flow fractionation device had differing expression patterns of EpCAM, indicating that affinity approaches reliant on EpCAM expression may underestimate CTC number and potentially miss critical subpopulations. Further characterization of CTCs, including low-EpCAM populations, using this technology may improve detection techniques and cancer diagnosis, ultimately improving cancer treatment.
UR - http://www.scopus.com/inward/record.url?scp=84966473543&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84966473543&partnerID=8YFLogxK
U2 - 10.18632/oncotarget.8250
DO - 10.18632/oncotarget.8250
M3 - Article
C2 - 27013581
AN - SCOPUS:84966473543
VL - 7
SP - 24677
EP - 24687
JO - Oncotarget
JF - Oncotarget
SN - 1949-2553
IS - 17
ER -