Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells

Dong Ha Kim, Mee Soo Chang, Chan Jin Yoon, Jaap M. Middeldorp, Olivia M. Martinez, Sun ju Byeon, Sun Young Rha, Sung Han Kim, Yang Soo Kim, Jun Hee Woo

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12 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV)-encoded BamHI-A rightward frame 1 (BARF1) is a putative viral oncogene in EBV-infected stomach cancer. The aim of the present study was to investigate BARF1-induced cellular protein and microRNA alterations. In this study, BARF1-expressing stomach cancer cells showed a high rate of proliferation, high levels of NFκB, and miR-146a upregulation, which was reversed by NFκB knockdown. During BARF1-induced NFκB upregulation, hCSF1 receptor level was unchanged. Knockdown of BARF1 in the naturally EBV-infected YCCEL1 stomach cancer cells suppressed cell proliferation, and downregulated NFκB and miR-146a. SMAD4 was identified as a miR-146a target and was downregulated in BARF1-expressing cells, whereas SMAD4 expression was restored by anti-miR-146a. Knockdown of BARF1 in YCCEL1 cells upregulated SMAD4, and this effect was reversed by miR-146a overexpression. Transfection of BARF1-expressing cells with pCEP4- SMAD4 abolished the cell proliferating effect of BARF1. In stomach cancer tissues, miR-146a was expressed at higher levels, and more frequent NFκB nuclear positivity immunohistochemically, but not of SMAD4 nuclear loss was found in the EBV-positive group compared with the EBV-negative group. In conclusion, EBV-encoded BARF1 promotes cell proliferation in stomach cancer by upregulating NFκB and miR-146a and downregulating SMAD4, thereby contributing to EBV-induced stomach cancer progression.

Original languageEnglish
Pages (from-to)82213-82227
Number of pages15
JournalOncotarget
Volume7
Issue number50
DOIs
Publication statusPublished - 2016 Jan 1

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Human Herpesvirus 4
Stomach Neoplasms
Down-Regulation
Up-Regulation
Cell Proliferation
MicroRNAs
Oncogenes
Transfection
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

Kim, D. H., Chang, M. S., Yoon, C. J., Middeldorp, J. M., Martinez, O. M., Byeon, S. J., ... Woo, J. H. (2016). Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells. Oncotarget, 7(50), 82213-82227. https://doi.org/10.18632/oncotarget.10511
Kim, Dong Ha ; Chang, Mee Soo ; Yoon, Chan Jin ; Middeldorp, Jaap M. ; Martinez, Olivia M. ; Byeon, Sun ju ; Rha, Sun Young ; Kim, Sung Han ; Kim, Yang Soo ; Woo, Jun Hee. / Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells. In: Oncotarget. 2016 ; Vol. 7, No. 50. pp. 82213-82227.
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abstract = "Epstein-Barr virus (EBV)-encoded BamHI-A rightward frame 1 (BARF1) is a putative viral oncogene in EBV-infected stomach cancer. The aim of the present study was to investigate BARF1-induced cellular protein and microRNA alterations. In this study, BARF1-expressing stomach cancer cells showed a high rate of proliferation, high levels of NFκB, and miR-146a upregulation, which was reversed by NFκB knockdown. During BARF1-induced NFκB upregulation, hCSF1 receptor level was unchanged. Knockdown of BARF1 in the naturally EBV-infected YCCEL1 stomach cancer cells suppressed cell proliferation, and downregulated NFκB and miR-146a. SMAD4 was identified as a miR-146a target and was downregulated in BARF1-expressing cells, whereas SMAD4 expression was restored by anti-miR-146a. Knockdown of BARF1 in YCCEL1 cells upregulated SMAD4, and this effect was reversed by miR-146a overexpression. Transfection of BARF1-expressing cells with pCEP4- SMAD4 abolished the cell proliferating effect of BARF1. In stomach cancer tissues, miR-146a was expressed at higher levels, and more frequent NFκB nuclear positivity immunohistochemically, but not of SMAD4 nuclear loss was found in the EBV-positive group compared with the EBV-negative group. In conclusion, EBV-encoded BARF1 promotes cell proliferation in stomach cancer by upregulating NFκB and miR-146a and downregulating SMAD4, thereby contributing to EBV-induced stomach cancer progression.",
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Kim, DH, Chang, MS, Yoon, CJ, Middeldorp, JM, Martinez, OM, Byeon, SJ, Rha, SY, Kim, SH, Kim, YS & Woo, JH 2016, 'Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells', Oncotarget, vol. 7, no. 50, pp. 82213-82227. https://doi.org/10.18632/oncotarget.10511

Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells. / Kim, Dong Ha; Chang, Mee Soo; Yoon, Chan Jin; Middeldorp, Jaap M.; Martinez, Olivia M.; Byeon, Sun ju; Rha, Sun Young; Kim, Sung Han; Kim, Yang Soo; Woo, Jun Hee.

In: Oncotarget, Vol. 7, No. 50, 01.01.2016, p. 82213-82227.

Research output: Contribution to journalArticle

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T1 - Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells

AU - Kim, Dong Ha

AU - Chang, Mee Soo

AU - Yoon, Chan Jin

AU - Middeldorp, Jaap M.

AU - Martinez, Olivia M.

AU - Byeon, Sun ju

AU - Rha, Sun Young

AU - Kim, Sung Han

AU - Kim, Yang Soo

AU - Woo, Jun Hee

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Epstein-Barr virus (EBV)-encoded BamHI-A rightward frame 1 (BARF1) is a putative viral oncogene in EBV-infected stomach cancer. The aim of the present study was to investigate BARF1-induced cellular protein and microRNA alterations. In this study, BARF1-expressing stomach cancer cells showed a high rate of proliferation, high levels of NFκB, and miR-146a upregulation, which was reversed by NFκB knockdown. During BARF1-induced NFκB upregulation, hCSF1 receptor level was unchanged. Knockdown of BARF1 in the naturally EBV-infected YCCEL1 stomach cancer cells suppressed cell proliferation, and downregulated NFκB and miR-146a. SMAD4 was identified as a miR-146a target and was downregulated in BARF1-expressing cells, whereas SMAD4 expression was restored by anti-miR-146a. Knockdown of BARF1 in YCCEL1 cells upregulated SMAD4, and this effect was reversed by miR-146a overexpression. Transfection of BARF1-expressing cells with pCEP4- SMAD4 abolished the cell proliferating effect of BARF1. In stomach cancer tissues, miR-146a was expressed at higher levels, and more frequent NFκB nuclear positivity immunohistochemically, but not of SMAD4 nuclear loss was found in the EBV-positive group compared with the EBV-negative group. In conclusion, EBV-encoded BARF1 promotes cell proliferation in stomach cancer by upregulating NFκB and miR-146a and downregulating SMAD4, thereby contributing to EBV-induced stomach cancer progression.

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Kim DH, Chang MS, Yoon CJ, Middeldorp JM, Martinez OM, Byeon SJ et al. Epstein-Barr virus BARF1-induced NFκB/miR-146a/SMAD4 alterations in stomach cancer cells. Oncotarget. 2016 Jan 1;7(50):82213-82227. https://doi.org/10.18632/oncotarget.10511