ERK activation is involved in tooth development via FGF10 signaling

Kyoung Won Cho, Jinglei Cai, Hyun Yi Kim, Akihiro Hosoya, Hayato Ohshima, Kang-Yell Choi, Hansung Jung

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The tooth is one of the ectodermal organs that develop from epithelial-mesenchymal interactions during embryonic development. An understanding of the underlying molecular mechanisms would improve our knowledge of the growth factors that regulate cell proliferation and differentiation. One of the related aspects is mitogen-activated protein kinase (MAPK) signaling in tooth differentiation. The extracellular-signal regulated kinase (ERK)/mitogen-activated protein kinase kinase (MEK) cascade plays a pivotal role in many of the essential cellular processes underlying embryonic development, including responses to major developmental changes. However, the role of the ERK pathway in molar development is unclear. This study investigated epithelial patterning and tooth growth in the mouse embryo by monitoring ERK and fibroblast growth factor (FGF) signaling. ERK, MEK, and phosphatase and tensin homolog (PTEN) were activated at different levels and locations in the developing tooth at E13.5 to E16.5 and PN2. ERK was activated in the inner dental epithelium and cervical loop, while PTEN was activated in the outer dental epithelium. In addition, only ERK was activated in secretory ameloblast at PN2. To further define the pathways involving FGF and ERK, tooth germs were cultured in the presence of compounds to inhibit MAPK/ERK-mediated signaling. Western blot analysis indicated that pERK2 was strongly activated in the tooth germ. Moreover, the activation level of pERK1 was dramatically increased by exogenous FGF10 alone and by combined treatment with FGF10 and U0126. The reported results will improve our understanding of the unique developmental processes of the dental epithelium and tooth growth, and will help to elucidate the fundamental mechanisms of ERK signaling underlying tooth development.

Original languageEnglish
Pages (from-to)901-911
Number of pages11
JournalJournal of Experimental Zoology Part B: Molecular and Developmental Evolution
Volume312
Issue number8
DOIs
Publication statusPublished - 2009 Dec 1

Fingerprint

Extracellular Signal-Regulated MAP Kinases
mitogen-activated protein kinase
tooth
Tooth
teeth
Tooth Germ
Epithelium
Fibroblast Growth Factors
Mitogen-Activated Protein Kinase Kinases
embryonic development
fibroblast growth factors
Mitogen-Activated Protein Kinases
Phosphoric Monoester Hydrolases
epithelium
phosphatase
protein
Embryonic Development
Ameloblasts
MAP Kinase Kinase Kinases
embryogenesis

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Medicine
  • Animal Science and Zoology
  • Genetics
  • Developmental Biology

Cite this

Cho, Kyoung Won ; Cai, Jinglei ; Kim, Hyun Yi ; Hosoya, Akihiro ; Ohshima, Hayato ; Choi, Kang-Yell ; Jung, Hansung. / ERK activation is involved in tooth development via FGF10 signaling. In: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 2009 ; Vol. 312, No. 8. pp. 901-911.
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ERK activation is involved in tooth development via FGF10 signaling. / Cho, Kyoung Won; Cai, Jinglei; Kim, Hyun Yi; Hosoya, Akihiro; Ohshima, Hayato; Choi, Kang-Yell; Jung, Hansung.

In: Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, Vol. 312, No. 8, 01.12.2009, p. 901-911.

Research output: Contribution to journalArticle

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AU - Kim, Hyun Yi

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