(Cell 165, 1507–1518; June 2, 2016) Our Resource paper reports the development of tools to image and perturb mechanical signaling pathways with fine spatiotemporal resolution. These tools were then used to interrogate spatial and mechanical response of Notch receptors and cadherin signaling. We recently found out that the plasmid encoding SNAP-Ecad-mEmerald, used to transfect U2OS cells to study F-actin dynamics in response to receptor segregation and mechanical loading of E-cadherin (Figure 5 of our paper), in fact encodes the VE-Cadherin, instead of E-Cadherin. While mislabeled, the VE-cadherin protein still serves the purpose of showing the unique capacity of our tool to manipulate receptor clustering and mechanical loading with high spatial and temporal resolution for mechanogenetic interrogation of cell surface receptors. The text and relevant figures in the online version of our paper have been corrected to reflect this change and three new references related to VE-cadherin were included in the discussion. We apologize for any inconvenience it may have caused to the scientific community.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)