Escherichia coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensor

Joachim Jose, Ji Won Chung, Byoung Jin Jeon, Ruth Maria Maas, Chang Hoon Nam, Jae Chul Pyun

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Generally, an immunoaffinity SPR biosensor detects a target analyte in a sample through highly selective adsorption by using the antigen-antibody interaction. For improving the sensitivity, various kinds of particles have been added to the already bound analytes on the SPR biosensor (sandwich assay). In this work, signal amplification was demonstrated by the expression of the IgG-binding Z-domain of protein A on the outer membrane of Escherichia coli via "Autodisplay". The amount of Z-domain of protein A expressed on the outer membrane was calculated to be 280,000 molecules per cell. In addition, the IgG-binding ability of the expressed protein was characterized using FACS analysis. The signal amplification of the SPR biosensor was performed in the sandwich assay format using a model of horseradish peroxidase (HRP); the limit of detection was determined to be significantly improved from 1 μg/ml to 1 ng/ml. Finally, myoglobin analysis was demonstrated for the medical diagnosis of cardiac diseases. The detection limit was estimated to be improved from 10 ng/ml to <1 ng/ml. These results show that Z-domain-displaying E. coli can be successfully used for the signal amplification of immunoaffinity biosensors, thereby improving the sensitivity and the limit of detection.

Original languageEnglish
Pages (from-to)1324-1329
Number of pages6
JournalBiosensors and Bioelectronics
Volume24
Issue number5
DOIs
Publication statusPublished - 2009 Jan 1

Fingerprint

Staphylococcal Protein A
Biosensing Techniques
Biosensors
Escherichia coli
Amplification
Proteins
Limit of Detection
Assays
Immunoglobulin G
Membranes
Myoglobin
Horseradish Peroxidase
Antigens
Antibodies
Adsorption
Heart Diseases
Molecules

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Jose, Joachim ; Chung, Ji Won ; Jeon, Byoung Jin ; Maas, Ruth Maria ; Nam, Chang Hoon ; Pyun, Jae Chul. / Escherichia coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensor. In: Biosensors and Bioelectronics. 2009 ; Vol. 24, No. 5. pp. 1324-1329.
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Escherichia coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensor. / Jose, Joachim; Chung, Ji Won; Jeon, Byoung Jin; Maas, Ruth Maria; Nam, Chang Hoon; Pyun, Jae Chul.

In: Biosensors and Bioelectronics, Vol. 24, No. 5, 01.01.2009, p. 1324-1329.

Research output: Contribution to journalArticle

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