Essential role of mitochondrial Ca2+ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells

Xianglan Quan; Tuyet Thi Nguyen; Seong Kyung Choi; Shanhua Xu; Ranjan Das; Seung Kuy Cha; Nari Kim; Jin Han; Andreas Wiederkehr; Claes B. Wollheim; Kyu Sang Park

doi:10.1074/jbc.M114.632547

Essential role of mitochondrial Ca²⁺ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells

Xianglan Quan, Tuyet Thi Nguyen, Seong Kyung Choi, Shanhua Xu, Ranjan Das, Seung Kuy Cha, Nari Kim, Jin Han, Andreas Wiederkehr, Claes B. Wollheim, Kyu Sang Park

Physiology

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Background: Mitochondrial Ca²⁺ uptake affects energy metabolism and insulin secretion. Results: Knockdown of mitochondrial Ca²⁺ uniporter decreases respiratory chain activity and mitochondrial pH gradient generation. Conclusion: Mitochondrial Ca²⁺ uptake via uniporter is essential for oxidative phosphorylation and metabolism-secretion coupling. Significance: The present study identifies mechanisms of action and bioenergetic consequences of mitochondrial Ca²⁺ transporters in insulin-releasing cells.

Original language	English
Pages (from-to)	4086-4096
Number of pages	11
Journal	Journal of Biological Chemistry
Volume	290
Issue number	7
DOIs	https://doi.org/10.1074/jbc.M114.632547
Publication status	Published - 2015 Feb 13

Bibliographical note

Publisher Copyright:
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1074/jbc.M114.632547

Cite this

Quan, X., Nguyen, T. T., Choi, S. K., Xu, S., Das, R., Cha, S. K., Kim, N., Han, J., Wiederkehr, A., Wollheim, C. B., & Park, K. S. (2015). Essential role of mitochondrial Ca²⁺ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells. Journal of Biological Chemistry, 290(7), 4086-4096. https://doi.org/10.1074/jbc.M114.632547

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Quan, X, Nguyen, TT, Choi, SK, Xu, S, Das, R, Cha, SK, Kim, N, Han, J, Wiederkehr, A, Wollheim, CB & Park, KS 2015, 'Essential role of mitochondrial Ca²⁺ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells', Journal of Biological Chemistry, vol. 290, no. 7, pp. 4086-4096. https://doi.org/10.1074/jbc.M114.632547

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AU - Nguyen, Tuyet Thi

AU - Choi, Seong Kyung

AU - Xu, Shanhua

AU - Das, Ranjan

AU - Cha, Seung Kuy

AU - Kim, Nari

AU - Han, Jin

AU - Wiederkehr, Andreas

AU - Wollheim, Claes B.

AU - Park, Kyu Sang

PY - 2015/2/13

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AB - Background: Mitochondrial Ca2+ uptake affects energy metabolism and insulin secretion. Results: Knockdown of mitochondrial Ca2+ uniporter decreases respiratory chain activity and mitochondrial pH gradient generation. Conclusion: Mitochondrial Ca2+ uptake via uniporter is essential for oxidative phosphorylation and metabolism-secretion coupling. Significance: The present study identifies mechanisms of action and bioenergetic consequences of mitochondrial Ca2+ transporters in insulin-releasing cells.

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