Essential role of mitochondrial permeability transition in vanilloid receptor 1-dependent cell death of sensory neurons

Chan Young Shin, Jieun Shin, Byungmoon Kim, Myeong Hyeon Wang, Jung Hee Jang, Young Joon Surh, Uhtaek Oh

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Capsaicin causes pain by activating VR1, a cloned capsaicin receptor, in sensory neurons. After the initial excitatory responses, capsaicin produces prolonged analgesia, presumably because of the neurotoxic effect that leads to the death of sensory neurons. However, the mechanism underlying capsaicin-induced cell death of sensory neurons is not known. Here we report that capsaicin induces cell death in VR1-expressing sensory neurons and VR1-transfected human embryonic kidney cells. Cell death of sensory neurons induced by capsaicin is accompanied by DNA fragmentation, TUNEL staining, and shrinkage of the nucleus in a caspase-dependent manner, indicating the apoptotic nature of the cell death. Mitochondrial permeability transition is likely to be a major component of capsaicin-induced cell death because bonkrekic acid and cyclosporin A, inhibitors of mitochondrial permeability transition, block this cell death. These results imply that capsaicin induces mitochondrial dysfunction in VR1-expressing cells, leading to apoptotic cell death, which is a well-known neurotoxic effect of capsaicin.

Original languageEnglish
Pages (from-to)57-68
Number of pages12
JournalMolecular and Cellular Neuroscience
Volume24
Issue number1
DOIs
Publication statusPublished - 2003 Sep 1

Fingerprint

Capsaicin
Sensory Receptor Cells
Permeability
Cell Death
TRPV Cation Channels
vanilloid receptor subtype 1
In Situ Nick-End Labeling
DNA Fragmentation
Caspases
Analgesia
Cyclosporine
Staining and Labeling
Kidney
Pain
Acids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Shin, Chan Young ; Shin, Jieun ; Kim, Byungmoon ; Wang, Myeong Hyeon ; Jang, Jung Hee ; Surh, Young Joon ; Oh, Uhtaek. / Essential role of mitochondrial permeability transition in vanilloid receptor 1-dependent cell death of sensory neurons. In: Molecular and Cellular Neuroscience. 2003 ; Vol. 24, No. 1. pp. 57-68.
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Essential role of mitochondrial permeability transition in vanilloid receptor 1-dependent cell death of sensory neurons. / Shin, Chan Young; Shin, Jieun; Kim, Byungmoon; Wang, Myeong Hyeon; Jang, Jung Hee; Surh, Young Joon; Oh, Uhtaek.

In: Molecular and Cellular Neuroscience, Vol. 24, No. 1, 01.09.2003, p. 57-68.

Research output: Contribution to journalArticle

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