Essential role of p53 in TPEN-induced neuronal apoptosis

Hana Ra, Hyun Lim Kim, Han Woong Lee, Yang Hee Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Depletion of intracellular zinc with N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) induces protein synthesis-dependent apoptosis. In this study, we examined the requirement for p53 as an upstream transcription factor in TPEN-induced neuronal apoptosis. Chemical or genetic blockade of p53 markedly attenuated TPEN-induced neuronal apoptosis, while the stability and activity of p53 were increased by TPEN. In addition, expression of proapoptotic genes, PUMA and NOXA, and activation of caspase-11 were increased by TPEN in a p53-dependent manner. Inhibition of p53 blocked cytochrome C release from mitochondria to cytosol and prevented caspase-3 activation. Therefore, p53 may be an essential regulatory factor for TPEN-induced neuronal apoptosis.

Original languageEnglish
Pages (from-to)1516-1520
Number of pages5
JournalFEBS Letters
Volume583
Issue number9
DOIs
Publication statusPublished - 2009 May 6

Fingerprint

ethylenediamine
Apoptosis
Chemical activation
Mitochondria
Cytochromes
Caspases
Caspase 3
Cytosol
Zinc
Transcription Factors
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Ra, Hana ; Kim, Hyun Lim ; Lee, Han Woong ; Kim, Yang Hee. / Essential role of p53 in TPEN-induced neuronal apoptosis. In: FEBS Letters. 2009 ; Vol. 583, No. 9. pp. 1516-1520.
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Essential role of p53 in TPEN-induced neuronal apoptosis. / Ra, Hana; Kim, Hyun Lim; Lee, Han Woong; Kim, Yang Hee.

In: FEBS Letters, Vol. 583, No. 9, 06.05.2009, p. 1516-1520.

Research output: Contribution to journalArticle

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