Establishment and characterization of a highly tumorigenic human diploid endometrial cancer cell line

Jin Woo Kim, Chun Geun Lee, Soo Kyung Choi, Jae Hoon Kim, Tae Eung Kim, Joon Mo Lee, Jong Gu Rha, Sung Eun Namkoong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A new cell line designated CUME-1 has been established from a poorly differentiated endometrial adenocarcinoma of the uterus. This cell line grew well without interruption for more than 88 months and 110 serial passages were successively carried out. The cells were highly tumorigenic in nude mice (85%). Repeated karyotype analyses from early (4th) to late (55th) passages of this cell line revealed a diploid stable clone in each passages without any noticeable structural or numerical aberrations. But from the 80th passage, a subpopulation with reciprocal transplantation between chromosomes 1q and 9q consistently appeared and was observed in about 30% of the cells. This cell line is one of the rare examples of experimentally proved tumorigenic cells of human solid tumor origin that retains the diploid karyotype in vitro. HLA typing indicated the presence of DR4, DR13, DQ3, and DQ6. Cytosol estrogen and progesterone receptors were found both in fresh primary tumor and in this cell line. Gonadotropin-releasing hormone (Gn-RH) receptor mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in cultured cells. Using the single-strand conformation polymorphism (SSCP) technique, we have screened CUME-1 cells for p53 mutation in exons 4 to 9. No mobility shift was observed. This cell line may be useful in studying the in vitro chromosomal evolution of the cell line and the in vivo properties of human endometrial adenocarcinoma.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalCancer genetics and cytogenetics
Volume99
Issue number1
DOIs
Publication statusPublished - 1997 Nov 1

Fingerprint

Endometrial Neoplasms
Diploidy
Cell Line
Karyotype
Adenocarcinoma
LHRH Receptors
Serial Passage
Histocompatibility Testing
Progesterone Receptors
Tumor Cell Line
Nude Mice
Estrogen Receptors
Cytosol
Reverse Transcription
Uterus
Exons
Cultured Cells
Clone Cells
Chromosomes
Transplantation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Kim, Jin Woo ; Lee, Chun Geun ; Choi, Soo Kyung ; Kim, Jae Hoon ; Kim, Tae Eung ; Lee, Joon Mo ; Rha, Jong Gu ; Namkoong, Sung Eun. / Establishment and characterization of a highly tumorigenic human diploid endometrial cancer cell line. In: Cancer genetics and cytogenetics. 1997 ; Vol. 99, No. 1. pp. 1-10.
@article{ee0e93f1fc0f4a9780609442678c12e8,
title = "Establishment and characterization of a highly tumorigenic human diploid endometrial cancer cell line",
abstract = "A new cell line designated CUME-1 has been established from a poorly differentiated endometrial adenocarcinoma of the uterus. This cell line grew well without interruption for more than 88 months and 110 serial passages were successively carried out. The cells were highly tumorigenic in nude mice (85{\%}). Repeated karyotype analyses from early (4th) to late (55th) passages of this cell line revealed a diploid stable clone in each passages without any noticeable structural or numerical aberrations. But from the 80th passage, a subpopulation with reciprocal transplantation between chromosomes 1q and 9q consistently appeared and was observed in about 30{\%} of the cells. This cell line is one of the rare examples of experimentally proved tumorigenic cells of human solid tumor origin that retains the diploid karyotype in vitro. HLA typing indicated the presence of DR4, DR13, DQ3, and DQ6. Cytosol estrogen and progesterone receptors were found both in fresh primary tumor and in this cell line. Gonadotropin-releasing hormone (Gn-RH) receptor mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in cultured cells. Using the single-strand conformation polymorphism (SSCP) technique, we have screened CUME-1 cells for p53 mutation in exons 4 to 9. No mobility shift was observed. This cell line may be useful in studying the in vitro chromosomal evolution of the cell line and the in vivo properties of human endometrial adenocarcinoma.",
author = "Kim, {Jin Woo} and Lee, {Chun Geun} and Choi, {Soo Kyung} and Kim, {Jae Hoon} and Kim, {Tae Eung} and Lee, {Joon Mo} and Rha, {Jong Gu} and Namkoong, {Sung Eun}",
year = "1997",
month = "11",
day = "1",
doi = "10.1016/S0165-4608(96)00389-5",
language = "English",
volume = "99",
pages = "1--10",
journal = "Cancer Genetics and Cytogenetics",
issn = "0165-4608",
publisher = "Elsevier Inc.",
number = "1",

}

Establishment and characterization of a highly tumorigenic human diploid endometrial cancer cell line. / Kim, Jin Woo; Lee, Chun Geun; Choi, Soo Kyung; Kim, Jae Hoon; Kim, Tae Eung; Lee, Joon Mo; Rha, Jong Gu; Namkoong, Sung Eun.

In: Cancer genetics and cytogenetics, Vol. 99, No. 1, 01.11.1997, p. 1-10.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Establishment and characterization of a highly tumorigenic human diploid endometrial cancer cell line

AU - Kim, Jin Woo

AU - Lee, Chun Geun

AU - Choi, Soo Kyung

AU - Kim, Jae Hoon

AU - Kim, Tae Eung

AU - Lee, Joon Mo

AU - Rha, Jong Gu

AU - Namkoong, Sung Eun

PY - 1997/11/1

Y1 - 1997/11/1

N2 - A new cell line designated CUME-1 has been established from a poorly differentiated endometrial adenocarcinoma of the uterus. This cell line grew well without interruption for more than 88 months and 110 serial passages were successively carried out. The cells were highly tumorigenic in nude mice (85%). Repeated karyotype analyses from early (4th) to late (55th) passages of this cell line revealed a diploid stable clone in each passages without any noticeable structural or numerical aberrations. But from the 80th passage, a subpopulation with reciprocal transplantation between chromosomes 1q and 9q consistently appeared and was observed in about 30% of the cells. This cell line is one of the rare examples of experimentally proved tumorigenic cells of human solid tumor origin that retains the diploid karyotype in vitro. HLA typing indicated the presence of DR4, DR13, DQ3, and DQ6. Cytosol estrogen and progesterone receptors were found both in fresh primary tumor and in this cell line. Gonadotropin-releasing hormone (Gn-RH) receptor mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in cultured cells. Using the single-strand conformation polymorphism (SSCP) technique, we have screened CUME-1 cells for p53 mutation in exons 4 to 9. No mobility shift was observed. This cell line may be useful in studying the in vitro chromosomal evolution of the cell line and the in vivo properties of human endometrial adenocarcinoma.

AB - A new cell line designated CUME-1 has been established from a poorly differentiated endometrial adenocarcinoma of the uterus. This cell line grew well without interruption for more than 88 months and 110 serial passages were successively carried out. The cells were highly tumorigenic in nude mice (85%). Repeated karyotype analyses from early (4th) to late (55th) passages of this cell line revealed a diploid stable clone in each passages without any noticeable structural or numerical aberrations. But from the 80th passage, a subpopulation with reciprocal transplantation between chromosomes 1q and 9q consistently appeared and was observed in about 30% of the cells. This cell line is one of the rare examples of experimentally proved tumorigenic cells of human solid tumor origin that retains the diploid karyotype in vitro. HLA typing indicated the presence of DR4, DR13, DQ3, and DQ6. Cytosol estrogen and progesterone receptors were found both in fresh primary tumor and in this cell line. Gonadotropin-releasing hormone (Gn-RH) receptor mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in cultured cells. Using the single-strand conformation polymorphism (SSCP) technique, we have screened CUME-1 cells for p53 mutation in exons 4 to 9. No mobility shift was observed. This cell line may be useful in studying the in vitro chromosomal evolution of the cell line and the in vivo properties of human endometrial adenocarcinoma.

UR - http://www.scopus.com/inward/record.url?scp=0030778799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030778799&partnerID=8YFLogxK

U2 - 10.1016/S0165-4608(96)00389-5

DO - 10.1016/S0165-4608(96)00389-5

M3 - Article

C2 - 9352788

AN - SCOPUS:0030778799

VL - 99

SP - 1

EP - 10

JO - Cancer Genetics and Cytogenetics

JF - Cancer Genetics and Cytogenetics

SN - 0165-4608

IS - 1

ER -