Establishment and characterization of mesenchymal stem cell-like clonal stem cells from mouse salivary glands

Jae Yol Lim, Tacghee Yi, Songyi Lee, Junghee Kim, Si Na Kim, Sun U. Song, Young Mo Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Successful therapy for radiation-induced salivary gland (SG) hypofunction is currently unavailable; however, tissue-specific stem cells are expected to be promising candidates for SG regeneration. Here, we present our method for the establishment of single cell-derived clonal stem cells from mouse SGs and describe their characteristics. Salivary gland-derived clonal stem cells (SGSCs) were isolated and expanded in vitro by a modified subfractionation culture method. The properties of SGSCs were examined with respect to their marker expression, gene expression, differentiation potential, and in vitro immunosuppressive activity relative to bone marrow-derived mesenchymal stem cells (BM-MSCs). SGSCs appeared to largely share the characteristics of BM-MSCs based on their marker expression, whereas they differentially expressed some genes, including AQP5, E-Cadherin, Laminin, ZO-1, and COL4. SGSCs showed the ability to differentiate into fat, bone, and cartilage cell types, as well as into α-amylase-producing and hepatocyte-like cells after appropriate induction. The in vitro immunosuppressive activity of SGSCs was found to be more potent than that of BM-MSCs. These results showed that SGSCs possess the properties of MSCs with some differential gene expression and they are salivary-specific stem cells with both epithelial and mesenchymal properties. The biological functions of SGSCs and their relevance to SG epithelial progenitor cells require further investigation.

Original languageEnglish
Pages (from-to)447-457
Number of pages11
JournalTissue Engineering - Part C: Methods
Volume21
Issue number5
DOIs
Publication statusPublished - 2015 May 1

Fingerprint

Salivary Glands
Stem cells
Mesenchymal Stromal Cells
Stem Cells
Bone
Bone Marrow
Immunosuppressive Agents
Gene expression
Gene Expression
Cadherins
Amylases
Cartilage
Laminin
Regeneration
Oils and fats
Hepatocytes
Cell culture
Radiotherapy
Epithelial Cells
Fats

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Lim, Jae Yol ; Yi, Tacghee ; Lee, Songyi ; Kim, Junghee ; Kim, Si Na ; Song, Sun U. ; Kim, Young Mo. / Establishment and characterization of mesenchymal stem cell-like clonal stem cells from mouse salivary glands. In: Tissue Engineering - Part C: Methods. 2015 ; Vol. 21, No. 5. pp. 447-457.
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Establishment and characterization of mesenchymal stem cell-like clonal stem cells from mouse salivary glands. / Lim, Jae Yol; Yi, Tacghee; Lee, Songyi; Kim, Junghee; Kim, Si Na; Song, Sun U.; Kim, Young Mo.

In: Tissue Engineering - Part C: Methods, Vol. 21, No. 5, 01.05.2015, p. 447-457.

Research output: Contribution to journalArticle

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