Establishment of a PF2D-MS/MS platform for rapid profiling and semiquantitative analysis of membrane protein biomarkers

Hyoung Joo Lee, Min Seok Kwon, Eun Young Lee, Yun Cho Sang, Young Ki Paik

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Current proteome profiling techniques have identified relatively few mammalian membrane proteins despite their numerous important functions. To establish a standard throughput-potential profiling platform for membrane proteins, Triton X-100-solubilized rat liver microsomal proteins were separated on a 2-D separation system (2-D liquid phase fractionation (PF2D)) in two different pH ranges (4.0-8.5 and 7.0-10.5). This system produced 182 proteins with more than two transmembrane domain (TMD), including 16 TMDs with high confidence. Comparative 2-D liquid maps with high resolution and reproducibility have been constructed for liver microsome from the phenobarbital (PB) treated rats. PF2D was also found to be useful for the semiquantification of some representative cytochrome P450 family proteins (e.g., cytochrome P450 2B2) that were induced by PB treatment compared with untreated controls. Thus, the combination of both high-detection capacity and rapid preliminary semiquantification in a PF2D platform could become a standard system for the routine analysis of membrane proteins.

Original languageEnglish
Pages (from-to)2168-2177
Number of pages10
JournalProteomics
Volume8
Issue number11
DOIs
Publication statusPublished - 2008 Jun 1

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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