Establishment of hydrochloric acid/lipopolysaccharide-induced pelvic inflammatory disease model

Yeonsu Oh, Jaehun Lee, Hyeon Cheol Kim, Tae Wook Hahn, Byung Il Yoon, Jeong Hee Han, Yong Soo Kwon, Joung Jun Park, Deog Bon Koo, Ki Jong Rhee, Bae Dong Jung

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Pelvic inflammatory disease (PID), which is one of the most problematic complications experienced by women with sexually transmitted diseases, frequently causes secondary infections after reproductive abnormalities in veterinary animals. Although the uterus is self-protective, it becomes fragile during periods or pregnancy. To investigate PID, bacteria or lipopolysaccharide (LPS) extracted from gram negative bacteria has been used to induce the disease in several animal models. However, when LPS is applied to the peritoneum, it often causes systemic sepsis leading to death and the PID was not consistently demonstrated. Hydrochloric acid (HCl) has been used to induce inflammation in the lungs and stomach but not tested for reproductive organs. In this study, we developed a PID model in mice by HCl and LPS sequential intracervical (i.c.) administration. The proinflammatory cytokines, interleukin (IL)-1β, IL-6 and tumor necrosis factor-α, were detected in the mouse uterus by western blot analysis and cytokine enzyme-linked immunosorbent assay after HCl (25 mg/kg) administration i.c. followed by four LPS (50 mg/kg) treatments. Moreover, mice exhibited increased infiltration of neutrophils in the endometrium and epithelial layer. These results suggest that ic co-administration of HCl and LPS induces PID in mice. This new model may provide a consistent and reproducible PID model for future research.

Original languageEnglish
Pages (from-to)413-419
Number of pages7
JournalJournal of veterinary science
Volume17
Issue number3
DOIs
Publication statusPublished - 2016 Sep 30

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Pelvic Inflammatory Disease
Hydrochloric Acid
disease models
hydrochloric acid
lipopolysaccharides
Lipopolysaccharides
mice
uterus
cytokines
Uterus
animal disease models
sexually transmitted diseases
peritoneum
sepsis (infection)
Cytokines
tumor necrosis factors
endometrium
interleukin-1
Gram-negative bacteria
Neutrophil Infiltration

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Oh, Y., Lee, J., Kim, H. C., Hahn, T. W., Yoon, B. I., Han, J. H., ... Jung, B. D. (2016). Establishment of hydrochloric acid/lipopolysaccharide-induced pelvic inflammatory disease model. Journal of veterinary science, 17(3), 413-419. https://doi.org/10.4142/jvs.2016.17.3.413
Oh, Yeonsu ; Lee, Jaehun ; Kim, Hyeon Cheol ; Hahn, Tae Wook ; Yoon, Byung Il ; Han, Jeong Hee ; Kwon, Yong Soo ; Park, Joung Jun ; Koo, Deog Bon ; Rhee, Ki Jong ; Jung, Bae Dong. / Establishment of hydrochloric acid/lipopolysaccharide-induced pelvic inflammatory disease model. In: Journal of veterinary science. 2016 ; Vol. 17, No. 3. pp. 413-419.
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abstract = "Pelvic inflammatory disease (PID), which is one of the most problematic complications experienced by women with sexually transmitted diseases, frequently causes secondary infections after reproductive abnormalities in veterinary animals. Although the uterus is self-protective, it becomes fragile during periods or pregnancy. To investigate PID, bacteria or lipopolysaccharide (LPS) extracted from gram negative bacteria has been used to induce the disease in several animal models. However, when LPS is applied to the peritoneum, it often causes systemic sepsis leading to death and the PID was not consistently demonstrated. Hydrochloric acid (HCl) has been used to induce inflammation in the lungs and stomach but not tested for reproductive organs. In this study, we developed a PID model in mice by HCl and LPS sequential intracervical (i.c.) administration. The proinflammatory cytokines, interleukin (IL)-1β, IL-6 and tumor necrosis factor-α, were detected in the mouse uterus by western blot analysis and cytokine enzyme-linked immunosorbent assay after HCl (25 mg/kg) administration i.c. followed by four LPS (50 mg/kg) treatments. Moreover, mice exhibited increased infiltration of neutrophils in the endometrium and epithelial layer. These results suggest that ic co-administration of HCl and LPS induces PID in mice. This new model may provide a consistent and reproducible PID model for future research.",
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Oh, Y, Lee, J, Kim, HC, Hahn, TW, Yoon, BI, Han, JH, Kwon, YS, Park, JJ, Koo, DB, Rhee, KJ & Jung, BD 2016, 'Establishment of hydrochloric acid/lipopolysaccharide-induced pelvic inflammatory disease model', Journal of veterinary science, vol. 17, no. 3, pp. 413-419. https://doi.org/10.4142/jvs.2016.17.3.413

Establishment of hydrochloric acid/lipopolysaccharide-induced pelvic inflammatory disease model. / Oh, Yeonsu; Lee, Jaehun; Kim, Hyeon Cheol; Hahn, Tae Wook; Yoon, Byung Il; Han, Jeong Hee; Kwon, Yong Soo; Park, Joung Jun; Koo, Deog Bon; Rhee, Ki Jong; Jung, Bae Dong.

In: Journal of veterinary science, Vol. 17, No. 3, 30.09.2016, p. 413-419.

Research output: Contribution to journalArticle

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AU - Oh, Yeonsu

AU - Lee, Jaehun

AU - Kim, Hyeon Cheol

AU - Hahn, Tae Wook

AU - Yoon, Byung Il

AU - Han, Jeong Hee

AU - Kwon, Yong Soo

AU - Park, Joung Jun

AU - Koo, Deog Bon

AU - Rhee, Ki Jong

AU - Jung, Bae Dong

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