Estrogenic activity of xanthorrhizol isolated from Curcuma xanthorrhiza ROXB

Anggakusuma, Yanti, Myoungsu Lee, Jae Kwan Hwang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Plant-derived estrogen-like compounds, or phytoestrogens, are given much attention due to their potential therapeutic use. In this study, xanthorrhizol, a natural sesquiterpenoid isolated from the rhizome of Curcuma xanthorrhiza ROXB. (Zingiberaceae), was evaluated for its estrogenic activity. It has been known that compounds acting as ligands for estrogen receptors (ERs) are considered to possess estrogenic activity. Therefore, the Gal-4/ER transactivation assay in transiently transfected African green monkey kidney (COS-7) cells was used to examine the estrogenic activity of xanthorrhizol. Both subtypes of ERs, ERα and ERβ, were involved in this assay. Further transactivation assays and pS2 mRNA analysis were also conducted in estrogen receptor-positive human breast cancer (MCF-7). Our results showed that xanthorrhizol significantly increased Gal-4/ER luciferase activity in a dose-dependent manner and induced the endogenous ER-estrogen response element (ERE) interaction in MCF-7 cells. Xanthorrhizol also significantly enhanced the expression of the pS2 gene in MCF-7 cells. In contrast, treatment using ICI 182780, an ER antagonist, suppressed all activities induced by xanthorrhizol, indicating ER-dependant activities were involved. These results suggest that xanthorrhizol possesses estrogenic activity and its estrogenic effects are mediated by estrogen-induced gene expression.

Original languageEnglish
Pages (from-to)1892-1897
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume32
Issue number11
DOIs
Publication statusPublished - 2009 Nov 1

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Curcuma
Estrogen Receptors
Phytoestrogens
Estrogens
MCF-7 Cells
Transcriptional Activation
Zingiberaceae
Gene Expression
xanthorrhizol
Cercopithecus aethiops
Rhizome
COS Cells
Response Elements
Therapeutic Uses
Luciferases
Breast Neoplasms
Ligands
Kidney
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

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abstract = "Plant-derived estrogen-like compounds, or phytoestrogens, are given much attention due to their potential therapeutic use. In this study, xanthorrhizol, a natural sesquiterpenoid isolated from the rhizome of Curcuma xanthorrhiza ROXB. (Zingiberaceae), was evaluated for its estrogenic activity. It has been known that compounds acting as ligands for estrogen receptors (ERs) are considered to possess estrogenic activity. Therefore, the Gal-4/ER transactivation assay in transiently transfected African green monkey kidney (COS-7) cells was used to examine the estrogenic activity of xanthorrhizol. Both subtypes of ERs, ERα and ERβ, were involved in this assay. Further transactivation assays and pS2 mRNA analysis were also conducted in estrogen receptor-positive human breast cancer (MCF-7). Our results showed that xanthorrhizol significantly increased Gal-4/ER luciferase activity in a dose-dependent manner and induced the endogenous ER-estrogen response element (ERE) interaction in MCF-7 cells. Xanthorrhizol also significantly enhanced the expression of the pS2 gene in MCF-7 cells. In contrast, treatment using ICI 182780, an ER antagonist, suppressed all activities induced by xanthorrhizol, indicating ER-dependant activities were involved. These results suggest that xanthorrhizol possesses estrogenic activity and its estrogenic effects are mediated by estrogen-induced gene expression.",
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Estrogenic activity of xanthorrhizol isolated from Curcuma xanthorrhiza ROXB. / Anggakusuma; Yanti; Lee, Myoungsu; Hwang, Jae Kwan.

In: Biological and Pharmaceutical Bulletin, Vol. 32, No. 11, 01.11.2009, p. 1892-1897.

Research output: Contribution to journalArticle

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