Ethyl-2, 5-dihydroxybenzoate displays dual activity by promoting osteoblast differentiation and inhibiting osteoclast differentiation

Byeong Ju Kwon, Mi Hee Lee, Min Ah Koo, Min Sung Kim, Gyeung Mi Seon, Jae Jin Han, Jongchul Park

Research output: Contribution to journalArticle

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Abstract

The interplay between bone-forming osteoblasts and bone-resorbing osteoclasts is essential for balanced bone remodeling. In this study, we evaluate the ability of ethyl-2, 5-dihyrdoxybenzoate (E-2, 5-DHB) to affect both osteoblast and osteoclast differentiation for bone regeneration. Osteogenic differentiation of human mesenchymal stem cells (hMSCs) was quantified by measuring alkaline phosphatase (ALP) activity and calcium deposition. To evaluate osteoclast differentiation, we investigated the effect of E-2, 5-DHB on RANKL-activated osteoclastogenesis in RAW 264.7 cells. E-2, 5-DHB enhanced ALP activity and inhibited RAW 264.7 cell osteoclastogenesis in vitro. To assess the in vivo activity of E-2, 5-DHB, hMSCs were delivered subcutaneosuly alone or in combination with E-2, 5-DHB in an alginate gel into the backs of nude-mice. Histological and immunohistochemical evaluation showed significantly higher calcium deposition in the E-2, 5-DHB group. Osteocalcin (OCN) was highly expressed in cells implanted in the gels containing E-2, 5-DHB. Our results suggest that E-2, 5-DHB can effectively enhance osteoblast differentiation and inhibit osteoclast differentiation both in vitro and in vivo. Understanding the dual function of E-2, 5-DHB on osteoblast and osteoclast differentiation will aid in future development of E-2, 5-DHB as a material for bone tissue engineering.

Original languageEnglish
Pages (from-to)335-341
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume471
Issue number3
DOIs
Publication statusPublished - 2016 Mar 11

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Osteoblasts
Osteoclasts
Bone
Stem cells
Mesenchymal Stromal Cells
Osteogenesis
Bone and Bones
Alkaline Phosphatase
Gels
Calcium
Bone Regeneration
Bone Remodeling
Osteocalcin
Tissue Engineering
Tissue engineering
Nude Mice
2,5-dihydroxybenzoic acid
RAW 264.7 Cells
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kwon, Byeong Ju ; Lee, Mi Hee ; Koo, Min Ah ; Kim, Min Sung ; Seon, Gyeung Mi ; Han, Jae Jin ; Park, Jongchul. / Ethyl-2, 5-dihydroxybenzoate displays dual activity by promoting osteoblast differentiation and inhibiting osteoclast differentiation. In: Biochemical and Biophysical Research Communications. 2016 ; Vol. 471, No. 3. pp. 335-341.
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Ethyl-2, 5-dihydroxybenzoate displays dual activity by promoting osteoblast differentiation and inhibiting osteoclast differentiation. / Kwon, Byeong Ju; Lee, Mi Hee; Koo, Min Ah; Kim, Min Sung; Seon, Gyeung Mi; Han, Jae Jin; Park, Jongchul.

In: Biochemical and Biophysical Research Communications, Vol. 471, No. 3, 11.03.2016, p. 335-341.

Research output: Contribution to journalArticle

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