Eukaryotic topoisomerase II cleavage of parallel stranded DNA tetraplexes

In Kwon Chung, V. B. Mehta, J. R. Spitzner, M. T. Muller

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A guanine-rich single-stranded DNA from the human immunoglobulin switch region was shown by Sen and Gilbert [Nature, (1988) 334, 364-366] to be able to self-associate to form a stable four-stranded parallel DNA structure. Topoisomerase II did not cleave the single-stranded DNA molecule. Surprisingly, the enzyme did cleave the same DNA sequence when it was annealed into the four-stranded structure. The two cleavage sites observed were the same as those found when this DNA molecule was paired with a complementary molecule to create a normal B-DNA duplex. These cleavages were shown to be protein-linked and reversible by the addition of salt, suggesting a normal topolsomerase II reaction mechanism. In addition, an eight-stranded DNA molecule created by the association of a complementary oligonucleotide with the four-stranded structure was also cleaved by topolsomerase II despite being resistant to restriction endonuclease digestion. These results suggest that a single strand of DNA may possess the sequence Information to direct topoisomerase II to a binding site, but the site must be base paired in a proper manner to do so. This demonstration of the ability of a four-stranded DNA molecule to be a substrate for an enzyme further suggests that these DNA structures may be present in cells.

Original languageEnglish
Pages (from-to)1973-1977
Number of pages5
JournalNucleic acids research
Volume20
Issue number8
DOIs
Publication statusPublished - 1992 Apr 25

Fingerprint

G-Quadruplexes
Type II DNA Topoisomerase
DNA
Molecules
Single-Stranded DNA
Enzymes
Immunoglobulin Switch Region
Immunoglobulin
Reaction Mechanism
B-Form DNA
Salt
DNA Sequence
DNA sequences
DNA Restriction Enzymes
Guanine
Switch
Oligonucleotides
Substrate
Digestion
Restriction

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Chung, In Kwon ; Mehta, V. B. ; Spitzner, J. R. ; Muller, M. T. / Eukaryotic topoisomerase II cleavage of parallel stranded DNA tetraplexes. In: Nucleic acids research. 1992 ; Vol. 20, No. 8. pp. 1973-1977.
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Eukaryotic topoisomerase II cleavage of parallel stranded DNA tetraplexes. / Chung, In Kwon; Mehta, V. B.; Spitzner, J. R.; Muller, M. T.

In: Nucleic acids research, Vol. 20, No. 8, 25.04.1992, p. 1973-1977.

Research output: Contribution to journalArticle

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