Evaluating the allergic risk of genetically modified soybean

Sangha Kim, Hyun Mi Kim, Young Min Ye, Seung Hyun Kim, Dong Ho Nahm, Hae Sim Park, Sang Ryeol Ryu, Bou Oung Lee

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Genetically modified (GM) soybean (carrying the EPSPS transgene) is the most common GM food in Korea. In order to assess whether genetic modification increases the allergenic risk of soybeans, the allergenicity and IgE-reactive components of wild-type and GM soybean extracts were compared in allergic adults who had been sensitized to soybeans. We enrolled 1,716 adult allergy patients and 40 healthy, non-atopic controls. Skin prick tests and IgF enzyme linked immunosorbent assays (ELISAs) were performed using wild-type and GM soybean extracts, along with other common inhaled allergens. The specifities of serum IgE antibodies from allergic patients and the identities of the IgE-reactive components of the soybean extracts were compared using ELISA inhibition testing, 2-Dimensional gel electrophoresis, and IgE immunoblotting. To evaluate the effects of digestive enzymes and heat treatment, the soybean extracts were heated or pre-incubated with or without simulated gastric and intestinal fluids. The IgE sensitization rates to Wild-type and GM soybeans were identical (3.8% of allergic adults), and circulating IgE antibodies specific for the two extracts were comparable. The results of the ELISA inhibition test, SDS-PAGE, and IgE immunoblotting showed a similar composition of IgE-binding components within the wild-type and GM extracts, which was confirmed using two-dimensional gel electrophoresis, IgE immunoblotting, and amino acid sequencing. None of the subjects had a positive response to purified EPSPS protein in the skin prick test. ELISA, or IgE immunoblot analysis. These findings suggest that the IgE sensitization rate to GM soybean extracts is identical to that of wild type soybean extracts in adult allergy patients. In addition, based in both in vivo and in vitro methods, the allergernicity of wild type and GM soybean extracts was identical.

Original languageEnglish
Pages (from-to)505-512
Number of pages8
JournalYonsei medical journal
Volume47
Issue number4
DOIs
Publication statusPublished - 2006 Jan 1

Fingerprint

Soybeans
Immunoglobulin E
Enzyme-Linked Immunosorbent Assay
Immunoblotting
Skin Tests
Hypersensitivity
Genetically Modified Food
Antibodies
Protein Sequence Analysis
Electrophoresis, Gel, Two-Dimensional
Korea
Transgenes
Allergens
Electrophoresis
Polyacrylamide Gel Electrophoresis
Stomach
Hot Temperature
Gels

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Kim, S., Kim, H. M., Ye, Y. M., Kim, S. H., Nahm, D. H., Park, H. S., ... Lee, B. O. (2006). Evaluating the allergic risk of genetically modified soybean. Yonsei medical journal, 47(4), 505-512. https://doi.org/10.3349/ymj.2006.47.4.505
Kim, Sangha ; Kim, Hyun Mi ; Ye, Young Min ; Kim, Seung Hyun ; Nahm, Dong Ho ; Park, Hae Sim ; Ryu, Sang Ryeol ; Lee, Bou Oung. / Evaluating the allergic risk of genetically modified soybean. In: Yonsei medical journal. 2006 ; Vol. 47, No. 4. pp. 505-512.
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Kim, S, Kim, HM, Ye, YM, Kim, SH, Nahm, DH, Park, HS, Ryu, SR & Lee, BO 2006, 'Evaluating the allergic risk of genetically modified soybean', Yonsei medical journal, vol. 47, no. 4, pp. 505-512. https://doi.org/10.3349/ymj.2006.47.4.505

Evaluating the allergic risk of genetically modified soybean. / Kim, Sangha; Kim, Hyun Mi; Ye, Young Min; Kim, Seung Hyun; Nahm, Dong Ho; Park, Hae Sim; Ryu, Sang Ryeol; Lee, Bou Oung.

In: Yonsei medical journal, Vol. 47, No. 4, 01.01.2006, p. 505-512.

Research output: Contribution to journalArticle

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