Evaluation of a fully automated treponemal test and comparison with conventional VDRL and FTA-ABS tests

Yongjung Park, Younhee Park, Shin Young Joo, Myoung Hee Park, Hyon Suk Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We evaluated analytic performances of an automated treponemal test and compared this test with the Venereal Disease Research Laboratory test (VDRL) and fluorescent treponemal antibody absorption test (FTA-ABS). Precision performance of the Architect Syphilis TP assay (TP; Abbott Japan, Tokyo, Japan) was assessed, and 150 serum samples were assayed with the TP before and after heat inactivation to estimate the effect of heat inactivation. A total of 616 specimens were tested with the FTA-ABS and TP, and 400 were examined with the VDRL. The TP showed good precision performance with total imprecision of less than a 10% coefficient of variation. An excellent linear relationship between results before and after heat inactivation was observed (R 2 = 0.9961). The FTA-ABS and TP agreed well with a &καππ α; coefficient of 0.981. The concordance rate between the FTA-ABS and TP was the highest (99.0%), followed by the rates between FTA-ABS and VDRL (85.0%) and between TP and VDRL (83.8%). The automated TP assay may be adequate for screening for syphilis in a large volume of samples and can be an alternative to FTA-ABS.

Original languageEnglish
Pages (from-to)705-710
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume136
Issue number5
DOIs
Publication statusPublished - 2011 Nov 1

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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