Evaluation of a rapid assay for identification of Mycobacterium tuberculosis grown in solid and liquid media

C. F. Ang, M. A.M. Cajucom, Y. Kim, H. Bang, Hyeyoung Lee, Sangnae Cho, C. S. Montalban

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

An MPT64 antigen detection assay, the SD Bioline TB Ag MPT64 Rapid kit, was evaluated for the identification of Mycobacterium tuberculosis grown in both solid and liquid media. The rapid test showed positive results in 132 of 133 (99.2%) M. tuberculosis cultures grown on Ogawa medium and 136/143 (95.1%) cultures grown in liquid culture medium, resulting in an overall sensitivity of 97.1%. All 18 non-tuberculous mycobacteria were found to be negative by the rapid test, indicating a specificity of 100%. The rapid test seems useful for the initial confirmation of M. tuberculosis in acidfast bacilli positive cultures.

Original languageEnglish
Pages (from-to)1475-1477
Number of pages3
JournalInternational Journal of Tuberculosis and Lung Disease
Volume15
Issue number11
DOIs
Publication statusPublished - 2011 Nov 1

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Mycobacterium tuberculosis
Mycobacterium
Bacillus
Culture Media
Antigens

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

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abstract = "An MPT64 antigen detection assay, the SD Bioline TB Ag MPT64 Rapid kit, was evaluated for the identification of Mycobacterium tuberculosis grown in both solid and liquid media. The rapid test showed positive results in 132 of 133 (99.2{\%}) M. tuberculosis cultures grown on Ogawa medium and 136/143 (95.1{\%}) cultures grown in liquid culture medium, resulting in an overall sensitivity of 97.1{\%}. All 18 non-tuberculous mycobacteria were found to be negative by the rapid test, indicating a specificity of 100{\%}. The rapid test seems useful for the initial confirmation of M. tuberculosis in acidfast bacilli positive cultures.",
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Evaluation of a rapid assay for identification of Mycobacterium tuberculosis grown in solid and liquid media. / Ang, C. F.; Cajucom, M. A.M.; Kim, Y.; Bang, H.; Lee, Hyeyoung; Cho, Sangnae; Montalban, C. S.

In: International Journal of Tuberculosis and Lung Disease, Vol. 15, No. 11, 01.11.2011, p. 1475-1477.

Research output: Contribution to journalArticle

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