Evaluation of an immunochromatographic assay kit for rapid identification of Mycobacterium tuberculosis complex in clinical isolates

Young Park Mi, Jin Kim Young, Hyun Hwang Sang, Hoi Kim Hyoung, Yup Lee Eun, Hoon Jeong Seok, Chulhun L. Chang

Research output: Contribution to journalArticle

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Abstract

We evaluated a new immunochromatographic assay (ICA) using mouse monoclonal anti-MPT64 antibody for rapid discrimination between Mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. A study with mycobacteria and other organisms showed excellent sensitivity (≅99%) and specificity (100%) and an appropriate detection limit (105 CFU/ml) when tested with M. tuberculosis H37Rv. This ICA can simplify the identification of M. tuberculosis in clinical laboratories.

Original languageEnglish
Pages (from-to)481-484
Number of pages4
JournalJournal of Clinical Microbiology
Volume47
Issue number2
DOIs
Publication statusPublished - 2009 Feb 1

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Immunochromatography
Mycobacterium tuberculosis
Nontuberculous Mycobacteria
Mycobacterium
Limit of Detection
Anti-Idiotypic Antibodies
Monoclonal Antibodies
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Mi, Young Park ; Young, Jin Kim ; Sang, Hyun Hwang ; Hyoung, Hoi Kim ; Eun, Yup Lee ; Seok, Hoon Jeong ; Chang, Chulhun L. / Evaluation of an immunochromatographic assay kit for rapid identification of Mycobacterium tuberculosis complex in clinical isolates. In: Journal of Clinical Microbiology. 2009 ; Vol. 47, No. 2. pp. 481-484.
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Evaluation of an immunochromatographic assay kit for rapid identification of Mycobacterium tuberculosis complex in clinical isolates. / Mi, Young Park; Young, Jin Kim; Sang, Hyun Hwang; Hyoung, Hoi Kim; Eun, Yup Lee; Seok, Hoon Jeong; Chang, Chulhun L.

In: Journal of Clinical Microbiology, Vol. 47, No. 2, 01.02.2009, p. 481-484.

Research output: Contribution to journalArticle

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