Evaluation of bio-hydrogen production using rice straw hydrolysate extracted by acid and alkali hydrolysis

Do Hyung Kim, In Seung Jo, Beom Jung Kang, Byung Don Lee, Sunil Kumar, Sang Hyoun Kim, Jeong Jun Yoon

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


This study was conducted to investigate the properties of hydrolysates obtained from acid and alkali hydrolysis and to evaluate the feasibility of employing them for bio-hydrogen production. High sugar concentrations of 16.8 g/L and 13.3 g/L were present in 0.5% and 1.0% H2SO4 hydrolysates, respectively. However, H2SO4 hydrolysis resulted in large amounts of short-chain fatty acids (SCFAs) and furan derivatives, which were removed by detoxification. In bio-hydrogen production, 1.0% H2SO4 hydrolysate showed a 55.6 mL of highest hydrogen production and 1.14 mol-H2/mol-hexose equivalentadded of hydrogen yield. In control and 1.0% NaOH hydrolysate, 29.7 mL and 36.9 mL of hydrogen were produced, respectively. Interestingly, relatively high acetate and butyrate production resulted in lactate reduction. Also, NH4OH hydrolysate produced less than 10 mL of hydrogen. Thus, these results indicate that hydrogen production and metabolite distribution can vary depending on the sugars and by-product composition in the hydrolysate.

Original languageEnglish
Pages (from-to)37385-37393
Number of pages9
JournalInternational Journal of Hydrogen Energy
Issue number88
Publication statusPublished - 2022 Oct 30

Bibliographical note

Funding Information:
This work was supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry and Energy (MOTIE) of the Republic of Korea ( 20188550000540 ).

Publisher Copyright:
© 2021 Hydrogen Energy Publications LLC

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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