Evaluation of cell proliferation in ear and lymph node using BrdU immunohistochemistry for mouse ear swelling test

Jong Kwon Lee, Jae Hyun Park, Hyung Soo Kim, Seung Tae Chung, Juno H. Eom, KiTaek Nam, Hye Young Oh

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Abstract

The mouse ear swelling test (MEST) was developed as an alternative to guinea pig models for measuring the contact sensitization potential. However, the MEST relies on the quantitative measurement of ear swelling by micrometer as the means of determining the endpoint. The purpose of this study was to investigate the possibility of using cell proliferation in the ear and lymph node by bromodeoxyuridine (BrdU) immunohistochemistry as a reliable marker for MEST. Female Balb/c mice were treated by the topical application of various sensitizers, 2,4-dinitrochlorobenzene (DNCB), toluene diisocyanate (TDI) and α-hexylcinnamaldehyde (HCA) and an irritant, sodium lauryl sulfate (SLS) following the protocol of MEST. The proliferation of cells in the ear and auricular lymph node was analyzed by BrdU incorporations into cells. There were significant increases in the cell proliferations of the ear and auricular lymph node in mice treated with DNCB and TDI compared to the vehicle control. All allergens and the irritant were correctly identified by the MEST using BrdU immunohistochemistry of lymph node responses. The standard MEST assay showed positive results in the case of the strong sensitizers, DNCB and TDI. However, HCA and SLS were not correctly identified in the ear swelling assay. These results suggest that the measurement of cell proliferation in the auricular lymph node using BrdU immunohistochemistry could provide a reliable marker for MEST.

Original languageEnglish
Pages (from-to)61-68
Number of pages8
JournalEnvironmental Toxicology and Pharmacology
Volume14
Issue number1-2
DOIs
Publication statusPublished - 2003 Jan 1

Fingerprint

Cell proliferation
Bromodeoxyuridine
Ear
Swelling
Lymph Nodes
Immunohistochemistry
Cell Proliferation
Toluene 2,4-Diisocyanate
Dinitrochlorobenzene
Irritants
Sodium Dodecyl Sulfate
Assays
Allergens
Guinea Pigs

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

Cite this

Lee, Jong Kwon ; Park, Jae Hyun ; Kim, Hyung Soo ; Chung, Seung Tae ; Eom, Juno H. ; Nam, KiTaek ; Oh, Hye Young. / Evaluation of cell proliferation in ear and lymph node using BrdU immunohistochemistry for mouse ear swelling test. In: Environmental Toxicology and Pharmacology. 2003 ; Vol. 14, No. 1-2. pp. 61-68.
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Evaluation of cell proliferation in ear and lymph node using BrdU immunohistochemistry for mouse ear swelling test. / Lee, Jong Kwon; Park, Jae Hyun; Kim, Hyung Soo; Chung, Seung Tae; Eom, Juno H.; Nam, KiTaek; Oh, Hye Young.

In: Environmental Toxicology and Pharmacology, Vol. 14, No. 1-2, 01.01.2003, p. 61-68.

Research output: Contribution to journalArticle

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