Evaluation of electrospun (1,3)-(1,6)-β-D-glucans/biodegradable polymer as artificial skin for full-thickness wound healing

Hye Lee Kim, Jeong Hyun Lee, Mi Hee Lee, Byeong Ju Kwon, Jongchul Park

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

(1,3)-(1,6)-β-D-glucan (BG), a natural product of glucose polymers, has immune stimulatory activity that is especially effective in wound healing. In this study, poly(lactic-co-glycolic acid) (PLGA) membranes containing BGs (BG/PLGA membranes) were investigated for their wound-healing effects. The growth rate of human dermal fibroblasts was enhanced in BG/PLGA membranes. Their growth rates were improved with the increase of BG concentration in the membranes. The PLGA membranes with and without BGs were treated in full-thickness skin wound using male BALB/c nude mice (n=6 for each group). According to the animal study, BG/PLGA membranes enhanced the interaction with the surrounding cells in wound sites. In the wound site treated BG/PLGA, the positive of the Ki-67 (a proliferation cell marker) and the CD 31 (an endothelial cell marker) were 77.2%±5.6% and 34±8.6 capillaries. In the wound site treated PLGA, the Ki-67 positive cells were 51.3%±7.0%, and the positive-stained capillaries of CD 31 were 22.7±8.6. The wound site treated with BG/PLGA membranes was stronger stained of them in the wound site than those of the wound sites treated with PLGA membranes. BG/PLGA membranes accelerated wound healing by improving the interaction, proliferation of cells, and angiogenesis. BG/PLGA membranes can be useful as a skin substitute for enhancing wound healing.

Original languageEnglish
Pages (from-to)2315-2322
Number of pages8
JournalTissue Engineering - Part A
Volume18
Issue number21-22
DOIs
Publication statusPublished - 2012 Nov 1

Fingerprint

Artificial Skin
Biodegradable polymers
Wound Healing
Skin
Polymers
Membranes
Acids
Wounds and Injuries
polylactic acid-polyglycolic acid copolymer
polyglucosan
Milk
Cell Proliferation
Glucans
Endothelial cells
Cell proliferation
Fibroblasts
Growth
Biological Products
Nude Mice
Cell Communication

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Kim, Hye Lee ; Lee, Jeong Hyun ; Lee, Mi Hee ; Kwon, Byeong Ju ; Park, Jongchul. / Evaluation of electrospun (1,3)-(1,6)-β-D-glucans/biodegradable polymer as artificial skin for full-thickness wound healing. In: Tissue Engineering - Part A. 2012 ; Vol. 18, No. 21-22. pp. 2315-2322.
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abstract = "(1,3)-(1,6)-β-D-glucan (BG), a natural product of glucose polymers, has immune stimulatory activity that is especially effective in wound healing. In this study, poly(lactic-co-glycolic acid) (PLGA) membranes containing BGs (BG/PLGA membranes) were investigated for their wound-healing effects. The growth rate of human dermal fibroblasts was enhanced in BG/PLGA membranes. Their growth rates were improved with the increase of BG concentration in the membranes. The PLGA membranes with and without BGs were treated in full-thickness skin wound using male BALB/c nude mice (n=6 for each group). According to the animal study, BG/PLGA membranes enhanced the interaction with the surrounding cells in wound sites. In the wound site treated BG/PLGA, the positive of the Ki-67 (a proliferation cell marker) and the CD 31 (an endothelial cell marker) were 77.2{\%}±5.6{\%} and 34±8.6 capillaries. In the wound site treated PLGA, the Ki-67 positive cells were 51.3{\%}±7.0{\%}, and the positive-stained capillaries of CD 31 were 22.7±8.6. The wound site treated with BG/PLGA membranes was stronger stained of them in the wound site than those of the wound sites treated with PLGA membranes. BG/PLGA membranes accelerated wound healing by improving the interaction, proliferation of cells, and angiogenesis. BG/PLGA membranes can be useful as a skin substitute for enhancing wound healing.",
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Evaluation of electrospun (1,3)-(1,6)-β-D-glucans/biodegradable polymer as artificial skin for full-thickness wound healing. / Kim, Hye Lee; Lee, Jeong Hyun; Lee, Mi Hee; Kwon, Byeong Ju; Park, Jongchul.

In: Tissue Engineering - Part A, Vol. 18, No. 21-22, 01.11.2012, p. 2315-2322.

Research output: Contribution to journalArticle

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T1 - Evaluation of electrospun (1,3)-(1,6)-β-D-glucans/biodegradable polymer as artificial skin for full-thickness wound healing

AU - Kim, Hye Lee

AU - Lee, Jeong Hyun

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N2 - (1,3)-(1,6)-β-D-glucan (BG), a natural product of glucose polymers, has immune stimulatory activity that is especially effective in wound healing. In this study, poly(lactic-co-glycolic acid) (PLGA) membranes containing BGs (BG/PLGA membranes) were investigated for their wound-healing effects. The growth rate of human dermal fibroblasts was enhanced in BG/PLGA membranes. Their growth rates were improved with the increase of BG concentration in the membranes. The PLGA membranes with and without BGs were treated in full-thickness skin wound using male BALB/c nude mice (n=6 for each group). According to the animal study, BG/PLGA membranes enhanced the interaction with the surrounding cells in wound sites. In the wound site treated BG/PLGA, the positive of the Ki-67 (a proliferation cell marker) and the CD 31 (an endothelial cell marker) were 77.2%±5.6% and 34±8.6 capillaries. In the wound site treated PLGA, the Ki-67 positive cells were 51.3%±7.0%, and the positive-stained capillaries of CD 31 were 22.7±8.6. The wound site treated with BG/PLGA membranes was stronger stained of them in the wound site than those of the wound sites treated with PLGA membranes. BG/PLGA membranes accelerated wound healing by improving the interaction, proliferation of cells, and angiogenesis. BG/PLGA membranes can be useful as a skin substitute for enhancing wound healing.

AB - (1,3)-(1,6)-β-D-glucan (BG), a natural product of glucose polymers, has immune stimulatory activity that is especially effective in wound healing. In this study, poly(lactic-co-glycolic acid) (PLGA) membranes containing BGs (BG/PLGA membranes) were investigated for their wound-healing effects. The growth rate of human dermal fibroblasts was enhanced in BG/PLGA membranes. Their growth rates were improved with the increase of BG concentration in the membranes. The PLGA membranes with and without BGs were treated in full-thickness skin wound using male BALB/c nude mice (n=6 for each group). According to the animal study, BG/PLGA membranes enhanced the interaction with the surrounding cells in wound sites. In the wound site treated BG/PLGA, the positive of the Ki-67 (a proliferation cell marker) and the CD 31 (an endothelial cell marker) were 77.2%±5.6% and 34±8.6 capillaries. In the wound site treated PLGA, the Ki-67 positive cells were 51.3%±7.0%, and the positive-stained capillaries of CD 31 were 22.7±8.6. The wound site treated with BG/PLGA membranes was stronger stained of them in the wound site than those of the wound sites treated with PLGA membranes. BG/PLGA membranes accelerated wound healing by improving the interaction, proliferation of cells, and angiogenesis. BG/PLGA membranes can be useful as a skin substitute for enhancing wound healing.

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