Evaluation of enzyme-linked immunosorbent assay using milk samples as a potential screening test of bovine tuberculosis of dairy cows in Korea

Bo Young Jeon, Seung Cheol Kim, Sungmo Je, Jeongyeon Kwak, Jang Eun Cho, Jong Tae Woo, Sangkyo Seo, Hang Sub Shim, Byoung Ok Park, Sung Sik Lee, Sang Nae Cho

Research output: Contribution to journalArticle

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Abstract

An enzyme-linked immunosorbent assay (ELISA) using bulk tank milk samples was evaluated as a screening test for bovine tuberculosis (TB), a contagious chronic disease of cattle. An ELISA with MPB70, a major antigen of Mycobacterium bovis was performed using paired sets of milk and sera samples from 33 tuberculin-positive and 43 tuberculin-negative cattle. Anti-MPB70 antibodies were detected in milk samples and there was a significant correlation between seroreactivities of milk and sera samples (R 2=0.83). Using the tuberculin skin test as the reference test, the sensitivities of ELISA using milk and sera samples were 87.8% and 81.8%, respectively, and the specificities were 97.7% and 100%, respectively.In the screening test using bulk tank milk samples from 931 dairy herds in Whasung, Gyeonggi-do, Korea, the positive rate for anti-MPB70 antibody was 4.5% (42/931) and the tuberculin-positive rate was 2.8% (26/931). Individual milk samples (n=253) were collected from randomly selected 8 problematic and 3 negative herds (positive and negative in the screening test by MPB70 ELISA using bulk tank milk samples, respectively) and tested by MPB70 milk ELISA. In the problematic herds, positive rates were 10.5% (20/190) for anti-MPB70 antibodies in milk ELISA and 2.1% (4/190) in the tuberculin skin test. More than one dairy cows were positive by milk ELISA among the problematic herds, and all tuberculin-positive dairy cows were positive in the milk ELISA. Further, no positive cows were detected in negative herds both by milk ELISA and tuberculin skin test. These results suggest that an ELISA, using bulk tank milk samples, might be a potential efficient screening test for bovine TB of dairy cows.

Original languageEnglish
Pages (from-to)390-393
Number of pages4
JournalResearch in Veterinary Science
Volume88
Issue number3
DOIs
Publication statusPublished - 2010 Jun 1

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Bovine Tuberculosis
bovine tuberculosis
Korea
Korean Peninsula
Milk
dairy cows
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
screening
milk
tuberculin
milk tanks
sampling
Tuberculin
Tuberculin Test
skin tests
Skin Tests
herds
Anti-Idiotypic Antibodies
antibodies

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

Jeon, Bo Young ; Kim, Seung Cheol ; Je, Sungmo ; Kwak, Jeongyeon ; Cho, Jang Eun ; Woo, Jong Tae ; Seo, Sangkyo ; Shim, Hang Sub ; Park, Byoung Ok ; Lee, Sung Sik ; Cho, Sang Nae. / Evaluation of enzyme-linked immunosorbent assay using milk samples as a potential screening test of bovine tuberculosis of dairy cows in Korea. In: Research in Veterinary Science. 2010 ; Vol. 88, No. 3. pp. 390-393.
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abstract = "An enzyme-linked immunosorbent assay (ELISA) using bulk tank milk samples was evaluated as a screening test for bovine tuberculosis (TB), a contagious chronic disease of cattle. An ELISA with MPB70, a major antigen of Mycobacterium bovis was performed using paired sets of milk and sera samples from 33 tuberculin-positive and 43 tuberculin-negative cattle. Anti-MPB70 antibodies were detected in milk samples and there was a significant correlation between seroreactivities of milk and sera samples (R 2=0.83). Using the tuberculin skin test as the reference test, the sensitivities of ELISA using milk and sera samples were 87.8{\%} and 81.8{\%}, respectively, and the specificities were 97.7{\%} and 100{\%}, respectively.In the screening test using bulk tank milk samples from 931 dairy herds in Whasung, Gyeonggi-do, Korea, the positive rate for anti-MPB70 antibody was 4.5{\%} (42/931) and the tuberculin-positive rate was 2.8{\%} (26/931). Individual milk samples (n=253) were collected from randomly selected 8 problematic and 3 negative herds (positive and negative in the screening test by MPB70 ELISA using bulk tank milk samples, respectively) and tested by MPB70 milk ELISA. In the problematic herds, positive rates were 10.5{\%} (20/190) for anti-MPB70 antibodies in milk ELISA and 2.1{\%} (4/190) in the tuberculin skin test. More than one dairy cows were positive by milk ELISA among the problematic herds, and all tuberculin-positive dairy cows were positive in the milk ELISA. Further, no positive cows were detected in negative herds both by milk ELISA and tuberculin skin test. These results suggest that an ELISA, using bulk tank milk samples, might be a potential efficient screening test for bovine TB of dairy cows.",
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Evaluation of enzyme-linked immunosorbent assay using milk samples as a potential screening test of bovine tuberculosis of dairy cows in Korea. / Jeon, Bo Young; Kim, Seung Cheol; Je, Sungmo; Kwak, Jeongyeon; Cho, Jang Eun; Woo, Jong Tae; Seo, Sangkyo; Shim, Hang Sub; Park, Byoung Ok; Lee, Sung Sik; Cho, Sang Nae.

In: Research in Veterinary Science, Vol. 88, No. 3, 01.06.2010, p. 390-393.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of enzyme-linked immunosorbent assay using milk samples as a potential screening test of bovine tuberculosis of dairy cows in Korea

AU - Jeon, Bo Young

AU - Kim, Seung Cheol

AU - Je, Sungmo

AU - Kwak, Jeongyeon

AU - Cho, Jang Eun

AU - Woo, Jong Tae

AU - Seo, Sangkyo

AU - Shim, Hang Sub

AU - Park, Byoung Ok

AU - Lee, Sung Sik

AU - Cho, Sang Nae

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N2 - An enzyme-linked immunosorbent assay (ELISA) using bulk tank milk samples was evaluated as a screening test for bovine tuberculosis (TB), a contagious chronic disease of cattle. An ELISA with MPB70, a major antigen of Mycobacterium bovis was performed using paired sets of milk and sera samples from 33 tuberculin-positive and 43 tuberculin-negative cattle. Anti-MPB70 antibodies were detected in milk samples and there was a significant correlation between seroreactivities of milk and sera samples (R 2=0.83). Using the tuberculin skin test as the reference test, the sensitivities of ELISA using milk and sera samples were 87.8% and 81.8%, respectively, and the specificities were 97.7% and 100%, respectively.In the screening test using bulk tank milk samples from 931 dairy herds in Whasung, Gyeonggi-do, Korea, the positive rate for anti-MPB70 antibody was 4.5% (42/931) and the tuberculin-positive rate was 2.8% (26/931). Individual milk samples (n=253) were collected from randomly selected 8 problematic and 3 negative herds (positive and negative in the screening test by MPB70 ELISA using bulk tank milk samples, respectively) and tested by MPB70 milk ELISA. In the problematic herds, positive rates were 10.5% (20/190) for anti-MPB70 antibodies in milk ELISA and 2.1% (4/190) in the tuberculin skin test. More than one dairy cows were positive by milk ELISA among the problematic herds, and all tuberculin-positive dairy cows were positive in the milk ELISA. Further, no positive cows were detected in negative herds both by milk ELISA and tuberculin skin test. These results suggest that an ELISA, using bulk tank milk samples, might be a potential efficient screening test for bovine TB of dairy cows.

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