Evaluation of fluorescence hs-CRP immunoassay for point-of-care testing

Wook Oh Sang, Dae Moon Jung, Yeol Park Sang, Jae Jang Heuk, Hoon Kim Jae, Bong Nahm Ki, Yul Choi Eui

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: C-reactive protein (CRP) is one of acute phase respondents that has been used to monitor infection and inflammation episodes. Recent studies have shown that high-sensitivity C-reactive protein (hs-CRP) is a potential risk predictor for future atherosclerosis and cardiovascular diseases (CVD). Methods: We previously developed a fluorescence-based immunochromatographic method for measuring hs-CRP concentrations (i-CHROMA™ hs-CRP assay) in blood. Whole blood was mixed with detector buffer, and then loaded onto a test cartridge. After 10 min of incubation, the test cartridge was inserted and scanned for acquisition of fluorescence intensity in a laser fluorescence reader (i-CHROMA™ reader). The fluorescence intensity was microprocessed and converted into the concentration of CRP in blood. The test result of 150 samples by the i-CHROMA™ hs-CRP assay method was compared and evaluated with those by TBA 200FR turbidimetry and BN II nephelometry method. The Deming regression and the Bland-Altman difference plot analysis were used for comparison of hs-CRP test result. Results: The i-CHROMA™ hs-CRP assay system exhibited a good linearity with in the whole measuring range (R = 0.997). The imprecision of intra- and the inter-assay CVs (coefficient of variation) of assay system were CVs< 3% and < 5% in the range of 0.5-20 mg/l, respectively. The i-CHROMA™ hs-CRP assay method correlated well with TBA 200FR turbidimetry and BN II nephelometry assay method (R = 0.988, N = 143 and R = 0.989, N = 143). Conclusion: The i-CHROMA™ hs-CRP assay system is comparable to those of other well-known fully automated hs-CRP assay and is suitable for point-of-care testing (POCT) in detection and quantification of hs-CRP.

Original languageEnglish
Pages (from-to)172-177
Number of pages6
JournalClinica Chimica Acta
Volume356
Issue number1-2
DOIs
Publication statusPublished - 2005 Jun 1

Fingerprint

Immunoassay
C-Reactive Protein
Fluorescence
Assays
Testing
Nephelometry and Turbidimetry
Blood
Point-of-Care Testing
Atherosclerosis
Buffers
Lasers
Cardiovascular Diseases
Inflammation
Detectors

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Sang, W. O., Jung, D. M., Sang, Y. P., Heuk, J. J., Jae, H. K., Ki, B. N., & Eui, Y. C. (2005). Evaluation of fluorescence hs-CRP immunoassay for point-of-care testing. Clinica Chimica Acta, 356(1-2), 172-177. https://doi.org/10.1016/j.cccn.2005.01.026
Sang, Wook Oh ; Jung, Dae Moon ; Sang, Yeol Park ; Heuk, Jae Jang ; Jae, Hoon Kim ; Ki, Bong Nahm ; Eui, Yul Choi. / Evaluation of fluorescence hs-CRP immunoassay for point-of-care testing. In: Clinica Chimica Acta. 2005 ; Vol. 356, No. 1-2. pp. 172-177.
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abstract = "Background: C-reactive protein (CRP) is one of acute phase respondents that has been used to monitor infection and inflammation episodes. Recent studies have shown that high-sensitivity C-reactive protein (hs-CRP) is a potential risk predictor for future atherosclerosis and cardiovascular diseases (CVD). Methods: We previously developed a fluorescence-based immunochromatographic method for measuring hs-CRP concentrations (i-CHROMA™ hs-CRP assay) in blood. Whole blood was mixed with detector buffer, and then loaded onto a test cartridge. After 10 min of incubation, the test cartridge was inserted and scanned for acquisition of fluorescence intensity in a laser fluorescence reader (i-CHROMA™ reader). The fluorescence intensity was microprocessed and converted into the concentration of CRP in blood. The test result of 150 samples by the i-CHROMA™ hs-CRP assay method was compared and evaluated with those by TBA 200FR turbidimetry and BN II nephelometry method. The Deming regression and the Bland-Altman difference plot analysis were used for comparison of hs-CRP test result. Results: The i-CHROMA™ hs-CRP assay system exhibited a good linearity with in the whole measuring range (R = 0.997). The imprecision of intra- and the inter-assay CVs (coefficient of variation) of assay system were CVs< 3{\%} and < 5{\%} in the range of 0.5-20 mg/l, respectively. The i-CHROMA™ hs-CRP assay method correlated well with TBA 200FR turbidimetry and BN II nephelometry assay method (R = 0.988, N = 143 and R = 0.989, N = 143). Conclusion: The i-CHROMA™ hs-CRP assay system is comparable to those of other well-known fully automated hs-CRP assay and is suitable for point-of-care testing (POCT) in detection and quantification of hs-CRP.",
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Sang, WO, Jung, DM, Sang, YP, Heuk, JJ, Jae, HK, Ki, BN & Eui, YC 2005, 'Evaluation of fluorescence hs-CRP immunoassay for point-of-care testing', Clinica Chimica Acta, vol. 356, no. 1-2, pp. 172-177. https://doi.org/10.1016/j.cccn.2005.01.026

Evaluation of fluorescence hs-CRP immunoassay for point-of-care testing. / Sang, Wook Oh; Jung, Dae Moon; Sang, Yeol Park; Heuk, Jae Jang; Jae, Hoon Kim; Ki, Bong Nahm; Eui, Yul Choi.

In: Clinica Chimica Acta, Vol. 356, No. 1-2, 01.06.2005, p. 172-177.

Research output: Contribution to journalArticle

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T1 - Evaluation of fluorescence hs-CRP immunoassay for point-of-care testing

AU - Sang, Wook Oh

AU - Jung, Dae Moon

AU - Sang, Yeol Park

AU - Heuk, Jae Jang

AU - Jae, Hoon Kim

AU - Ki, Bong Nahm

AU - Eui, Yul Choi

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N2 - Background: C-reactive protein (CRP) is one of acute phase respondents that has been used to monitor infection and inflammation episodes. Recent studies have shown that high-sensitivity C-reactive protein (hs-CRP) is a potential risk predictor for future atherosclerosis and cardiovascular diseases (CVD). Methods: We previously developed a fluorescence-based immunochromatographic method for measuring hs-CRP concentrations (i-CHROMA™ hs-CRP assay) in blood. Whole blood was mixed with detector buffer, and then loaded onto a test cartridge. After 10 min of incubation, the test cartridge was inserted and scanned for acquisition of fluorescence intensity in a laser fluorescence reader (i-CHROMA™ reader). The fluorescence intensity was microprocessed and converted into the concentration of CRP in blood. The test result of 150 samples by the i-CHROMA™ hs-CRP assay method was compared and evaluated with those by TBA 200FR turbidimetry and BN II nephelometry method. The Deming regression and the Bland-Altman difference plot analysis were used for comparison of hs-CRP test result. Results: The i-CHROMA™ hs-CRP assay system exhibited a good linearity with in the whole measuring range (R = 0.997). The imprecision of intra- and the inter-assay CVs (coefficient of variation) of assay system were CVs< 3% and < 5% in the range of 0.5-20 mg/l, respectively. The i-CHROMA™ hs-CRP assay method correlated well with TBA 200FR turbidimetry and BN II nephelometry assay method (R = 0.988, N = 143 and R = 0.989, N = 143). Conclusion: The i-CHROMA™ hs-CRP assay system is comparable to those of other well-known fully automated hs-CRP assay and is suitable for point-of-care testing (POCT) in detection and quantification of hs-CRP.

AB - Background: C-reactive protein (CRP) is one of acute phase respondents that has been used to monitor infection and inflammation episodes. Recent studies have shown that high-sensitivity C-reactive protein (hs-CRP) is a potential risk predictor for future atherosclerosis and cardiovascular diseases (CVD). Methods: We previously developed a fluorescence-based immunochromatographic method for measuring hs-CRP concentrations (i-CHROMA™ hs-CRP assay) in blood. Whole blood was mixed with detector buffer, and then loaded onto a test cartridge. After 10 min of incubation, the test cartridge was inserted and scanned for acquisition of fluorescence intensity in a laser fluorescence reader (i-CHROMA™ reader). The fluorescence intensity was microprocessed and converted into the concentration of CRP in blood. The test result of 150 samples by the i-CHROMA™ hs-CRP assay method was compared and evaluated with those by TBA 200FR turbidimetry and BN II nephelometry method. The Deming regression and the Bland-Altman difference plot analysis were used for comparison of hs-CRP test result. Results: The i-CHROMA™ hs-CRP assay system exhibited a good linearity with in the whole measuring range (R = 0.997). The imprecision of intra- and the inter-assay CVs (coefficient of variation) of assay system were CVs< 3% and < 5% in the range of 0.5-20 mg/l, respectively. The i-CHROMA™ hs-CRP assay method correlated well with TBA 200FR turbidimetry and BN II nephelometry assay method (R = 0.988, N = 143 and R = 0.989, N = 143). Conclusion: The i-CHROMA™ hs-CRP assay system is comparable to those of other well-known fully automated hs-CRP assay and is suitable for point-of-care testing (POCT) in detection and quantification of hs-CRP.

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