Evaluation of Meibum Lipid Composition According to Tear Interferometric Patterns: RRH: Meibum Composition According to Interferometric Patterns

Ikhyun Jun, Sehui Kim, Hyoseon Kim, Sun Woong Kim, Yong Woo Ji, Kwang Pyo Kim, Tai Gyu Lee, Kyoung Yul Seo

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2 Citations (Scopus)


Purpose: To determine the relationship between tear film interferometric patterns and properties of lipid, including rheological properties. Design: Prospective, cross-sectional laboratory investigation. Method: This study included 105 subjects (94 dry eye patients and 11 normal participants). The subjects were divided into 3 categories (group 1, normal; group 2, thin; and group 3, irregular) according to interferometric patterns. According to tear interferometric patterns, ultra-performance liquid chromatography (LC) quadrupole-linear ion trap/mass spectrometry (MS)-based analysis was used to investigate lipid profiling of meibum. Rheological properties were examined by using a Langmuir-Blodgett trough with saline solution. Results: Normal subjects showed Pearl-like patterns, and dry eye patients showed either irregular or thin patterns. Group 2 tended to be the evaporative type, and group 3 tended to be the aqueous-deficient type. Lipid profiling using LC-MS identified 280 lipid species of 25 lipid classes. In the meibum of the patient groups, the content of cholesteryl esters and nonpolar lipids was lower than that in the normal group. However, the content of polar lipids such as sphingolipids and phospholipids in the patient groups was higher than that in the normal group. Rheological properties showed that the lift-off areas were comparable among the 3 groups and the surface tension was the highest in group 1, followed by group 3 and group 2. Conclusions: The findings of this study suggest that tear interferometric patterns are associated with lipid profiling of meibum and its rheological properties. These results may contribute toward the development of new treatment modalities.

Original languageEnglish
Pages (from-to)37-50
Number of pages14
JournalAmerican Journal of Ophthalmology
Publication statusPublished - 2022 Aug

Bibliographical note

Funding Information:
Funding: This work was supported by the Basic Science Research Program (NRF- 2021R1I1A1A01047951 and NRF-2019R1F1A1063311), and Korea Mouse Phenotyping Project (NRF-2013M3A9D5072551) of the National Research Foundation funded by the Ministry of Science, ICT, and Future Planning. The funding sources had no role in the design or conduct of the study; collection, management, analysis, or interpretation of the data; preparation, review, or approval of the manuscript; or the decision to submit the manuscript for publication. Financial Disclosure: None of the authors has proprietary or financial interests regarding the materials and methods used in this study. All authors attest that they meet the current ICMJE criteria for authorship.

Publisher Copyright:
© 2022 Elsevier Inc.

All Science Journal Classification (ASJC) codes

  • Ophthalmology


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