Evaluation of mycobacterial interspersed repetitive unit-variable number tandem repeat typing to discriminate mycobacterium tuberculosis strains from myanmar

Phyu Win Ei, Wah Wah Aung, Wint Wint Nyunt, Thyn Lei Swe, Si Thu Aung, Mi Mi Htwe, Aye Su Mon, Su Mon Win, Chulhun L. Chang, Hyeyoung Lee, Jong Seok Lee

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Abstract

Mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing is a fast and promising method to discriminate Mycobacterium tuberculosis (MTB) strains. The present study was carried out to evaluate the applicability of MIRU-VNTR method for genotyping of clinical MTB strains from new pulmonary TB patients in Myanmar, and to compare the discriminatory ability between 15 loci and 24 loci MIRU-VNTR typing. One hundred and seven clinical MTB isolates which were collected during 2012-2013 were examined for anti-TB drug susceptibility using both proportion method and commercial line-probe assay. The results of internationally standardized 15 loci and 24 loci MIRU-VNTR typing methods were analyzed by a MIRU-VNTR plus web application together with Hunter-Gaston discriminatory index (HGDI) as numerical index to describe the discriminatory power. All tested MTB isolates showed unique patterns, which did not cluster and were distributed among 14 lineages. EAI genotype (30%) was the most prevalent genotype, followed by Beijing genotype (29%) that is significantly associated with multidrug-resistant TB (p <0.0004). Both 15 and 24 loci MIRU-VNTR were highly discriminatory (HGDI= 0.9833 and 0.9874, respectively). This study indicates that MIRU-VNTR could be used as a genotyping tool for Myanmar MTB strains.

Original languageEnglish
Pages (from-to)276-284
Number of pages9
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume49
Issue number2
Publication statusPublished - 2018 Mar 1

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All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Ei, P. W., Aung, W. W., Nyunt, W. W., Swe, T. L., Aung, S. T., Htwe, M. M., Mon, A. S., Win, S. M., Chang, C. L., Lee, H., & Lee, J. S. (2018). Evaluation of mycobacterial interspersed repetitive unit-variable number tandem repeat typing to discriminate mycobacterium tuberculosis strains from myanmar. Southeast Asian Journal of Tropical Medicine and Public Health, 49(2), 276-284.