Evaluation of PCR-reverse blot hybridization assay, REBA sepsis-ID test, for simultaneous identification of bacterial pathogens and mecA and van genes from blood culture bottles

Soon Deok Park, Gyusang Lee, Hye Young Wang, Min Park, Sunghyun Kim, Hyunjung Kim, Jungho Kim, Young Keun Kim, Hyo Youl Kim, Hyeyoung Lee, Young Uh, Jong Bae Kim

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5 Citations (Scopus)

Abstract

Background: The aim of this study was to evaluate a newly developed PCR-based reverse blot hybridization assay (PCR-REBA), REBA Sepsis-ID (M&D, Wonju, Korea), to rapidly detect the presence of bacteremia and antimicrobial resistance gene in blood culture samples. Methods: One thousand four hundred consecutive blood culture samples from patients with a delta neutrophil index greater than 2.7% were selected from March to July in 2013. Three hundred positive and 1,100 negative for bacterial growth in blood culture bottles samples were tested by conventional and real-time PCR-REBA, respectively. Results: The overall agreement between the conventional identification test and the REBA Sepsis-ID test was 95.3% (286/300). Agreement for gram-positive bacteria, gram-negative bacteria, fungi, and polymicrobials was 94.5% (190/201), 97.3% (71/73), 100% (14/14), and 91.7% (11/12), respectively. The detection rate of the mecA gene from methicillin-resistant Staphylococcus isolates was 97.8% (90/92). The vanA gene was detected in one blood culture sample from which vancomycin-resistant Enterococcus was isolated. When the cycle threshold for real-time PCR was defined as 30.0, 2.4% (26/1,100) of negative blood culture samples tested positive by real-time PCR. Conclusions: The REBA Sepsis-ID test is capable of simultaneously and quickly detecting both causative agents and antimicrobial resistance genes, such as mecA and van, in blood culture positive samples.

Original languageEnglish
Pages (from-to)446-455
Number of pages10
JournalAnnals of laboratory medicine
Volume34
Issue number6
DOIs
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

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