Evaluation of phenotypic screening methods for detecting plasmid-mediated AmpC β-lactamases-producing isolates of Escherichia coli and Klebsiella pneumoniae

Kyungwon Lee, Seong Geun Hong, Yeon Joon Park, Hye Soo Lee, Wonkeun Song, Joseph Jeong, Dongeun Yong, Yunsop Chong

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Detection of plasmid-mediated (P-M) AmpC β-lactamase-producing isolates is considered critical for epidemiologic studies and hospital infection control, but the documents of the Clinical and Laboratory Standards Institute do not contain any recommendation for the phenotypic detection. In this study, phenotypic detection methods, cefoxitin-Hodge test and induction test, were evaluated using cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae isolates. The cefoxitin-Hodge test detected all blaCMY-10, and 97.4% of blaCMY-2 allele-positive isolates, but only 57.3% of bla DHA-1 allele-positive isolates. Induction test with an aztreonam and an amoxicillin-clavulanic acid disk was more sensitive than with cefoxitin disk, which detected 86.6% of blaDHA-1 allele-positive isolates. These phenotypic tests should be useful to screen P-M AmpC β-lactamase-producing E. coli and K. pneumoniae isolates.

Original languageEnglish
Pages (from-to)319-323
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume53
Issue number4
DOIs
Publication statusPublished - 2005 Dec 1

Fingerprint

Cefoxitin
Klebsiella pneumoniae
Plasmids
Escherichia coli
Alleles
Aztreonam
Amoxicillin-Potassium Clavulanate Combination
Infection Control
Cross Infection
Epidemiologic Studies

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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abstract = "Detection of plasmid-mediated (P-M) AmpC β-lactamase-producing isolates is considered critical for epidemiologic studies and hospital infection control, but the documents of the Clinical and Laboratory Standards Institute do not contain any recommendation for the phenotypic detection. In this study, phenotypic detection methods, cefoxitin-Hodge test and induction test, were evaluated using cefoxitin-resistant Escherichia coli and Klebsiella pneumoniae isolates. The cefoxitin-Hodge test detected all blaCMY-10, and 97.4{\%} of blaCMY-2 allele-positive isolates, but only 57.3{\%} of bla DHA-1 allele-positive isolates. Induction test with an aztreonam and an amoxicillin-clavulanic acid disk was more sensitive than with cefoxitin disk, which detected 86.6{\%} of blaDHA-1 allele-positive isolates. These phenotypic tests should be useful to screen P-M AmpC β-lactamase-producing E. coli and K. pneumoniae isolates.",
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Evaluation of phenotypic screening methods for detecting plasmid-mediated AmpC β-lactamases-producing isolates of Escherichia coli and Klebsiella pneumoniae. / Lee, Kyungwon; Hong, Seong Geun; Park, Yeon Joon; Lee, Hye Soo; Song, Wonkeun; Jeong, Joseph; Yong, Dongeun; Chong, Yunsop.

In: Diagnostic Microbiology and Infectious Disease, Vol. 53, No. 4, 01.12.2005, p. 319-323.

Research output: Contribution to journalArticle

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