Evaluation of revisited fucosylated alpha-fetoprotein (AFP-L3) with an autoanalyzer μTAS in a clinical laboratory

Jonghyeon Choi, Yongjung Park, Jeong Ho Kim, Hyon Suk Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: We assessed clinical and analytical performances of AFP and fucosylated AFP (AFP-L3) assays by a newly developed automated analyzer based on liquid-phase binding (micro-total analysis systems, μTAS). Methods: A total of 239 serum samples were obtained from 120 patients with hepatocellular carcinoma (HCC) and 119 without HCC. Precision of assays by the μTAS was evaluated, and the correlation between AFP-L3 and AFP levels was analyzed. Receiver operating characteristics curve-area under the curve (ROC-AUC) value was calculated to measure the diagnostic performance. Results: Imprecision for AFP (ng/ml), AFP-L3 (ng/ml), and AFP-L3 (%) with 2 levels of QC materials was all within 5% coefficient of variation. AFP levels measured by the μTAS were correlated well with those by the UniCel DxI 800 Access (r=0.83). AFP-L3 concentrations in HCC patients were higher than those in control group (median 379.2. ng/ml in HCC, 1.0. ng/ml in non-HCC, P<0.05). AUC of AFP-L3 was 0.91 which was significantly higher than that of AFP (0.88 by μTAS; 0.84 by UniCel DxI 800, P<0.05 for both). Conclusion: AFP-L3 in HCC was significantly higher than that of control group. The μTAS showed good performances for routine uses in clinical laboratories for measuring AFP and AFP-L3.

Original languageEnglish
Pages (from-to)170-174
Number of pages5
JournalClinica Chimica Acta
Volume413
Issue number1-2
DOIs
Publication statusPublished - 2012 Jan 18

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Clinical laboratories
alpha-Fetoproteins
Hepatocellular Carcinoma
Assays
Area Under Curve
Liquids
Control Groups
ROC Curve
Carcinoma
Serum

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

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title = "Evaluation of revisited fucosylated alpha-fetoprotein (AFP-L3) with an autoanalyzer μTAS in a clinical laboratory",
abstract = "Background: We assessed clinical and analytical performances of AFP and fucosylated AFP (AFP-L3) assays by a newly developed automated analyzer based on liquid-phase binding (micro-total analysis systems, μTAS). Methods: A total of 239 serum samples were obtained from 120 patients with hepatocellular carcinoma (HCC) and 119 without HCC. Precision of assays by the μTAS was evaluated, and the correlation between AFP-L3 and AFP levels was analyzed. Receiver operating characteristics curve-area under the curve (ROC-AUC) value was calculated to measure the diagnostic performance. Results: Imprecision for AFP (ng/ml), AFP-L3 (ng/ml), and AFP-L3 ({\%}) with 2 levels of QC materials was all within 5{\%} coefficient of variation. AFP levels measured by the μTAS were correlated well with those by the UniCel DxI 800 Access (r=0.83). AFP-L3 concentrations in HCC patients were higher than those in control group (median 379.2. ng/ml in HCC, 1.0. ng/ml in non-HCC, P<0.05). AUC of AFP-L3 was 0.91 which was significantly higher than that of AFP (0.88 by μTAS; 0.84 by UniCel DxI 800, P<0.05 for both). Conclusion: AFP-L3 in HCC was significantly higher than that of control group. The μTAS showed good performances for routine uses in clinical laboratories for measuring AFP and AFP-L3.",
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Evaluation of revisited fucosylated alpha-fetoprotein (AFP-L3) with an autoanalyzer μTAS in a clinical laboratory. / Choi, Jonghyeon; Park, Yongjung; Kim, Jeong Ho; Kim, Hyon Suk.

In: Clinica Chimica Acta, Vol. 413, No. 1-2, 18.01.2012, p. 170-174.

Research output: Contribution to journalArticle

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N2 - Background: We assessed clinical and analytical performances of AFP and fucosylated AFP (AFP-L3) assays by a newly developed automated analyzer based on liquid-phase binding (micro-total analysis systems, μTAS). Methods: A total of 239 serum samples were obtained from 120 patients with hepatocellular carcinoma (HCC) and 119 without HCC. Precision of assays by the μTAS was evaluated, and the correlation between AFP-L3 and AFP levels was analyzed. Receiver operating characteristics curve-area under the curve (ROC-AUC) value was calculated to measure the diagnostic performance. Results: Imprecision for AFP (ng/ml), AFP-L3 (ng/ml), and AFP-L3 (%) with 2 levels of QC materials was all within 5% coefficient of variation. AFP levels measured by the μTAS were correlated well with those by the UniCel DxI 800 Access (r=0.83). AFP-L3 concentrations in HCC patients were higher than those in control group (median 379.2. ng/ml in HCC, 1.0. ng/ml in non-HCC, P<0.05). AUC of AFP-L3 was 0.91 which was significantly higher than that of AFP (0.88 by μTAS; 0.84 by UniCel DxI 800, P<0.05 for both). Conclusion: AFP-L3 in HCC was significantly higher than that of control group. The μTAS showed good performances for routine uses in clinical laboratories for measuring AFP and AFP-L3.

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