Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing

Y. Park, YuSeun Kim, S. I. Kim, H. Kim, Hyonsuk Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The human leukocyte antigen (HLA)-B*51 genotype is one of the well-known genetic factors associated with the development of Behcet's disease. We evaluated three sequence-specific priming (SSP) assays and one real-time PCR assay for detecting HLA-B*51 alleles using 93 whole blood samples, which were genotyped by high-resolution sequence-based typing (SBT). All HLA-B*51 alleles determined by SBT were detected by the four evaluated assays, and the results for all HLA-B alleles other than HLA-B*51 were negative on all assays. Thus, all HLA-B51 tests showed 100% sensitivity and 100% specificity for detecting HLA-B*51 alleles. The three SSP assays and the real-time PCR test for HLA-B*51 genotyping are simple, but reliable for detecting HLA-B*51 alleles in clinical laboratories.

Original languageEnglish
Pages (from-to)376-379
Number of pages4
JournalTissue Antigens
Volume80
Issue number4
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

Polymerase chain reaction
HLA Antigens
Real-Time Polymerase Chain Reaction
Assays
Alleles
Clinical laboratories
Behcet Syndrome
Blood
Genotype
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

Cite this

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abstract = "The human leukocyte antigen (HLA)-B*51 genotype is one of the well-known genetic factors associated with the development of Behcet's disease. We evaluated three sequence-specific priming (SSP) assays and one real-time PCR assay for detecting HLA-B*51 alleles using 93 whole blood samples, which were genotyped by high-resolution sequence-based typing (SBT). All HLA-B*51 alleles determined by SBT were detected by the four evaluated assays, and the results for all HLA-B alleles other than HLA-B*51 were negative on all assays. Thus, all HLA-B51 tests showed 100{\%} sensitivity and 100{\%} specificity for detecting HLA-B*51 alleles. The three SSP assays and the real-time PCR test for HLA-B*51 genotyping are simple, but reliable for detecting HLA-B*51 alleles in clinical laboratories.",
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Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing. / Park, Y.; Kim, YuSeun; Kim, S. I.; Kim, H.; Kim, Hyonsuk.

In: Tissue Antigens, Vol. 80, No. 4, 01.10.2012, p. 376-379.

Research output: Contribution to journalArticle

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AU - Kim, Hyonsuk

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