Evaluation of the cobas U 701 microscopy analyser compared with urine culture in screening for urinary tract infection

Si Hyun Kim, Sae Am Song, Sang Hwa Urm, Joong Ki Kook, Hye Ran Kim, DongEun Yong, JunYong Choi, Jeong Hwan Shin

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Abstract

Purpose. A new automated Cobas u 701 microscopy analyser for urine sediment examination was introduced. The aim of this study was to evaluate the analyser in comparison with urine culture in screening for urinary tract infection (UTI). Methodology. A total of 852 urine specimens submitted for culture were included in this study. Urine sediment examination was performed using the Cobas u 701 microscopy analyser. The results of the bacteria (BAC) and yeast (YEA) analyses were compared with the results from urine culture as a method for UTI screening. In addition, we compared the BAC results with white blood cells (WBCs) and leukocyte and nitrite measurement in the Cobas u 601 system. Results. Of the 852 urine specimens, 16.1% (N=137) were positive by urine culture, yielding 130 bacteria from 124 specimens and 14 yeasts from 14 specimens. The Cobas u 701 microscopy analyser provided no result for 52 specimens because of their high turbidity. The sensitivity, specificity, positive predictive value and negative predictive value were 85.8, 69.4, 33.1 and 96.5%, respectively. For YEA, these figures were 100, 91.9, 15.8 and 100%, respectively. The areas under the curve for BAC and WBCs were 0.827 [95% confidence interval (CI) 0.799, 0.852] and 0.727 (95% CI 0.695, 0.757), respectively. The sensitivity of the leukocyte and nitrite was 63.5 and 54.6%, respectively. Conclusion. The Cobas u 701 microscopy analyser showed good diagnostic performance. It can be used for rapid screening for UTI and can reduce the number of cultures required.

Original languageEnglish
Pages (from-to)1110-1113
Number of pages4
JournalJournal of Medical Microbiology
Volume66
Issue number8
DOIs
Publication statusPublished - 2017 Aug 1

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Urinary Tract Infections
Microscopy
Urine
Leukocytes
Bacteria
Yeasts
Nitrites
Confidence Intervals
Area Under Curve
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

Kim, Si Hyun ; Song, Sae Am ; Urm, Sang Hwa ; Kook, Joong Ki ; Kim, Hye Ran ; Yong, DongEun ; Choi, JunYong ; Shin, Jeong Hwan. / Evaluation of the cobas U 701 microscopy analyser compared with urine culture in screening for urinary tract infection. In: Journal of Medical Microbiology. 2017 ; Vol. 66, No. 8. pp. 1110-1113.
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title = "Evaluation of the cobas U 701 microscopy analyser compared with urine culture in screening for urinary tract infection",
abstract = "Purpose. A new automated Cobas u 701 microscopy analyser for urine sediment examination was introduced. The aim of this study was to evaluate the analyser in comparison with urine culture in screening for urinary tract infection (UTI). Methodology. A total of 852 urine specimens submitted for culture were included in this study. Urine sediment examination was performed using the Cobas u 701 microscopy analyser. The results of the bacteria (BAC) and yeast (YEA) analyses were compared with the results from urine culture as a method for UTI screening. In addition, we compared the BAC results with white blood cells (WBCs) and leukocyte and nitrite measurement in the Cobas u 601 system. Results. Of the 852 urine specimens, 16.1{\%} (N=137) were positive by urine culture, yielding 130 bacteria from 124 specimens and 14 yeasts from 14 specimens. The Cobas u 701 microscopy analyser provided no result for 52 specimens because of their high turbidity. The sensitivity, specificity, positive predictive value and negative predictive value were 85.8, 69.4, 33.1 and 96.5{\%}, respectively. For YEA, these figures were 100, 91.9, 15.8 and 100{\%}, respectively. The areas under the curve for BAC and WBCs were 0.827 [95{\%} confidence interval (CI) 0.799, 0.852] and 0.727 (95{\%} CI 0.695, 0.757), respectively. The sensitivity of the leukocyte and nitrite was 63.5 and 54.6{\%}, respectively. Conclusion. The Cobas u 701 microscopy analyser showed good diagnostic performance. It can be used for rapid screening for UTI and can reduce the number of cultures required.",
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Evaluation of the cobas U 701 microscopy analyser compared with urine culture in screening for urinary tract infection. / Kim, Si Hyun; Song, Sae Am; Urm, Sang Hwa; Kook, Joong Ki; Kim, Hye Ran; Yong, DongEun; Choi, JunYong; Shin, Jeong Hwan.

In: Journal of Medical Microbiology, Vol. 66, No. 8, 01.08.2017, p. 1110-1113.

Research output: Contribution to journalArticle

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AU - Song, Sae Am

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AU - Kook, Joong Ki

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AU - Yong, DongEun

AU - Choi, JunYong

AU - Shin, Jeong Hwan

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N2 - Purpose. A new automated Cobas u 701 microscopy analyser for urine sediment examination was introduced. The aim of this study was to evaluate the analyser in comparison with urine culture in screening for urinary tract infection (UTI). Methodology. A total of 852 urine specimens submitted for culture were included in this study. Urine sediment examination was performed using the Cobas u 701 microscopy analyser. The results of the bacteria (BAC) and yeast (YEA) analyses were compared with the results from urine culture as a method for UTI screening. In addition, we compared the BAC results with white blood cells (WBCs) and leukocyte and nitrite measurement in the Cobas u 601 system. Results. Of the 852 urine specimens, 16.1% (N=137) were positive by urine culture, yielding 130 bacteria from 124 specimens and 14 yeasts from 14 specimens. The Cobas u 701 microscopy analyser provided no result for 52 specimens because of their high turbidity. The sensitivity, specificity, positive predictive value and negative predictive value were 85.8, 69.4, 33.1 and 96.5%, respectively. For YEA, these figures were 100, 91.9, 15.8 and 100%, respectively. The areas under the curve for BAC and WBCs were 0.827 [95% confidence interval (CI) 0.799, 0.852] and 0.727 (95% CI 0.695, 0.757), respectively. The sensitivity of the leukocyte and nitrite was 63.5 and 54.6%, respectively. Conclusion. The Cobas u 701 microscopy analyser showed good diagnostic performance. It can be used for rapid screening for UTI and can reduce the number of cultures required.

AB - Purpose. A new automated Cobas u 701 microscopy analyser for urine sediment examination was introduced. The aim of this study was to evaluate the analyser in comparison with urine culture in screening for urinary tract infection (UTI). Methodology. A total of 852 urine specimens submitted for culture were included in this study. Urine sediment examination was performed using the Cobas u 701 microscopy analyser. The results of the bacteria (BAC) and yeast (YEA) analyses were compared with the results from urine culture as a method for UTI screening. In addition, we compared the BAC results with white blood cells (WBCs) and leukocyte and nitrite measurement in the Cobas u 601 system. Results. Of the 852 urine specimens, 16.1% (N=137) were positive by urine culture, yielding 130 bacteria from 124 specimens and 14 yeasts from 14 specimens. The Cobas u 701 microscopy analyser provided no result for 52 specimens because of their high turbidity. The sensitivity, specificity, positive predictive value and negative predictive value were 85.8, 69.4, 33.1 and 96.5%, respectively. For YEA, these figures were 100, 91.9, 15.8 and 100%, respectively. The areas under the curve for BAC and WBCs were 0.827 [95% confidence interval (CI) 0.799, 0.852] and 0.727 (95% CI 0.695, 0.757), respectively. The sensitivity of the leukocyte and nitrite was 63.5 and 54.6%, respectively. Conclusion. The Cobas u 701 microscopy analyser showed good diagnostic performance. It can be used for rapid screening for UTI and can reduce the number of cultures required.

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