Evaluation of the diagnostic utility of a whole-blood interferon-γ assay for determining the risk of exposure to Mycobacterium tuberculosis in Bacille Calmette-Guerin (BCG)-vaccinated individuals

Seok Yong Eum, Ye Jin Lee, Hyun Kyung Kwak, Jin Hong Min, Soo Hee Hwang, Laura E. Via, Clifton E. Barry, Sangnae Cho

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We evaluated the utility of the "QuantiFERON®-TB Gold In-Tube" (QuantiFERON®) test that uses tuberculosis (TB)-specific antigens for the diagnosis of latent infection in such individuals. We also examined the correlation between the interferon (IFN)-γ response to these antigens and the exposure risk to TB by evaluating antigen-specific IFN-γ release in comparison with IFN-γ release in response to purified protein derivative (PPD) in 3 groups: medical students, nurses in a TB hospital, and TB patients. All nurses and TB patients responded to PPD, whereas 52% (P < 0.0001) and 79.2% (P = 0.04) responded to QuantiFERON®, respectively. In the medical students, only 10.4% responded to QuantiFERON®, whereas 85.2% were positive to PPD (P < 0.0001). There was also a significant correlation between the levels of IFN-γ production and the duration of employment in the group of nurses at the TB hospital, suggesting ongoing exposure in this high-risk group. Thus, these results demonstrate that Mycobacterium tuberculosis-specific IFN-γ release assay accurately discriminates low- and high-risk healthy subjects and might therefore be a useful diagnostic tool for the diagnosis of latent infection in Bacille Calmette-Guerin (BCG)-vaccinated individuals.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume61
Issue number2
DOIs
Publication statusPublished - 2008 Jun 1

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Mycobacterium tuberculosis
Interferons
Tuberculosis
Chronic Disease Hospitals
Nurses
Medical Students
Antigens
Proteins
Infection
Gold
Healthy Volunteers

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "We evaluated the utility of the {"}QuantiFERON{\circledR}-TB Gold In-Tube{"} (QuantiFERON{\circledR}) test that uses tuberculosis (TB)-specific antigens for the diagnosis of latent infection in such individuals. We also examined the correlation between the interferon (IFN)-γ response to these antigens and the exposure risk to TB by evaluating antigen-specific IFN-γ release in comparison with IFN-γ release in response to purified protein derivative (PPD) in 3 groups: medical students, nurses in a TB hospital, and TB patients. All nurses and TB patients responded to PPD, whereas 52{\%} (P < 0.0001) and 79.2{\%} (P = 0.04) responded to QuantiFERON{\circledR}, respectively. In the medical students, only 10.4{\%} responded to QuantiFERON{\circledR}, whereas 85.2{\%} were positive to PPD (P < 0.0001). There was also a significant correlation between the levels of IFN-γ production and the duration of employment in the group of nurses at the TB hospital, suggesting ongoing exposure in this high-risk group. Thus, these results demonstrate that Mycobacterium tuberculosis-specific IFN-γ release assay accurately discriminates low- and high-risk healthy subjects and might therefore be a useful diagnostic tool for the diagnosis of latent infection in Bacille Calmette-Guerin (BCG)-vaccinated individuals.",
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Evaluation of the diagnostic utility of a whole-blood interferon-γ assay for determining the risk of exposure to Mycobacterium tuberculosis in Bacille Calmette-Guerin (BCG)-vaccinated individuals. / Eum, Seok Yong; Lee, Ye Jin; Kwak, Hyun Kyung; Min, Jin Hong; Hwang, Soo Hee; Via, Laura E.; Barry, Clifton E.; Cho, Sangnae.

In: Diagnostic Microbiology and Infectious Disease, Vol. 61, No. 2, 01.06.2008, p. 181-186.

Research output: Contribution to journalArticle

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