Evaluation of the effect of expansion and shear stress on a self-assembled endothelium mimicking nanomatrix coating for drug eluting stents in vitro and in vivo

Adinarayana Andukuri, Iljae Min, Patrick Hwang, Grant Alexander, Lauren E. Marshall, Joel L. Berry, Timothy M. Wick, Yoon Ki Joung, Young Sup Yoon, Brigitta C. Brott, Dong Keun Han, Ho Wook Jun

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8 Citations (Scopus)

Abstract

Coating stability is increasingly recognized as a concern impacting the long-term effectiveness of drug eluting stents (DES). In particular, unstable coatings have been brought into focus by a recently published report (Denardo et al 2012 J. Am. Med. Assoc. 307 2148-50). Towards the goal of overcoming current challenges of DES performance, we have developed an endothelium mimicking nanomatrix coating composed of peptide amphiphiles that promote endothelialization, but limit smooth muscle cell proliferation and platelet adhesion. Here, we report a novel water evaporation based method to uniformly coat the endothelium mimicking nanomatrix onto stents using a rotational coating technique, thereby eliminating residual chemicals and organic solvents, and allowing easy application to even bioabsorbable stents. Furthermore, the stability of the endothelium mimicking nanomatrix was analyzed after force experienced during expansion and shear stress under simulated physiological conditions. Results demonstrate uniformity and structural integrity of the nanomatrix coating. Preliminary animal studies in a rabbit model showed no flaking or peeling, and limited neointimal formation or restenosis. Therefore, it has the potential to improve the clinical performance of DES by providing multifunctional endothelium mimicking characteristics with structural integrity on stent surfaces.

Original languageEnglish
Article number35019
JournalBiofabrication
Volume6
Issue number3
DOIs
Publication statusPublished - 2014

Bibliographical note

Funding Information:
This study was supported by NIH (1R03EB017344-01) for HWJ, NIH (1DP3DK094346-01) for HWJ and YY, and AHA predoctoral fellowship for AA. It was also supported by the Cell Regeneration Program (2012M3A9C6049717) through the NRF of Korea funded by the MSIP, Advanced Medical New Material (Fiber) Development Program (2MR1390) founded by MOTIE, and KIST Program (2E24680) founded by MSIP, Korea for DKH and YKJ. We would also like to thank Dr Derrick Dean's lab for providing access to FTIR equipment.

Funding Information:
This study was supported by NIH (1R03EB017344-01) for HWJ, NIH (1DP3DK094346-01) for HWJ and YY, and AHA predoctoral fellowship for AA. It was also supported by the Cell Regeneration Program (2012M3A9C6049717) through the NRF of Korea funded by the MSIP, Advanced Medical New Material (Fiber) Development Program (2MR1390) founded by MOTIE, and KIST Program (2E24680) founded by MSIP, Korea for DKH and YKJ. We would also like to thank Dr Derrick Deanʼs lab for providing access to FTIR equipment.

Publisher Copyright:
© 2014 IOP Publishing Ltd Printed in the UK.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

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